TEMPERATURE-CONTROLLED RELEASE OF AN AQUEOUS MARKER FROM LIPOSOMES COATED WITHNIPAM-ODA COPOLYMERS

doi:10.7517/j.issn.1674-0475.1999.03.208

Abstract

Abstract: To study the temperature-controlled release behavior of the liposome coated with an amphiphilic copolymer which is temperaturesensitive, the copolymer of N-isopropylacrylamide (NIPAM) and octadecyl acrylate (ODA) was synthesized. The phenomenon of LCST(Lower Critical Solution Temperature)of the copolymer in aqueous solution with the increase of the temperature was studied by using fluorescence probe method. Results showed that the obvious phase separation occurs when temperature was increased to 30. The release property of small unilamellar vesicles (SUV) coated with the copolymer was studied by using 5(6)-carboxyfluorescein (5(6)-CF) as a water soluble marker. Results showed that the coating of the copolymer on the liposomal surface resulted in reduction of the release below 30 and enhancement of the release above 30. It indicated that the temperature-controlled release behavior of the liposome was directly related to the phase separation behavior of the coating copolymer when temperature was increased to its LSCT. Moreover, fluorescence polarization method was used to study the influence of the coating copolymer on the fluidity of the liposomal membrane. Results showed that at temperature below 30 the fluidity of the liposomal membrane increased with the increase of temperature; however, at temperature above 30 the fluidity of the liposomal membrane decreased with the increase of temperature. It further indicated that the copolymer which was bound at the surface of the liposome can destroy the liposomal membrane when temperature was increased at or above its LCST.

Key words: water-soluble polymer, liposomes, temperature-controlled release, fluorescence polarization

SHI Xiangyang, SUN Caomin, WU Shikang. TEMPERATURE-CONTROLLED RELEASE OF AN AQUEOUS MARKER FROM LIPOSOMES COATED WITHNIPAM-ODA COPOLYMERS[J]. Imaging Science and Photochemistry, 1999, 17(3): 208-216.

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