Abstract: In present work the molecular weight distribution (MWD) of inert gelatins and modified gelatins was studied by High Performance Gel Permeation Chromatography. Three columns of μ-Bondagel E-linear was connected in series and used for separation. Sodium dodecyl sulfate (2%) was used as eluent. The α, β and γ components were used as standard samples. The M_N, M_W, M_Z and M_η of inert and modified gelatins were measured and compared with the calculated values. The degree of degradation of the parent gelatin during it’s modification process under high pH was determined and the results were discussed.