血卟啉衍生物光照产生超氧化物阴离子自由基的一些观察

doi:10.7517/j.issn.1674-0475.1991.03.191

影像科学与光化学 ›› 1991, Vol. 9 ›› Issue (3): 191-197.DOI: 10.7517/j.issn.1674-0475.1991.03.191

血卟啉衍生物光照产生超氧化物阴离子自由基的一些观察

曹恩华, 辛淑敏

中国科学院生物物理研究所, 北京 100080

收稿日期:1989-11-09 修回日期:1990-08-21 出版日期:1991-08-20 发布日期:1991-08-20
通讯作者: 曹恩华
基金资助:
国家自然科学基金

SOME OBSERVATION ON THE SUPEROXIDE RADICAL FORMATION BY HEMATOPORPHYRIN DERIVATIVE PHOTOSENSITIZATION

CAO EN-HUA, XIN SHU-MIN

Institute of Biophysics, Acodcmia Sinica, Beijing 100080, P. R. China

Received:1989-11-09 Revised:1990-08-21 Online:1991-08-20 Published:1991-08-20

摘要/Abstract

摘要： 用氮蓝四唑(NBT)、细胞色素C还原及DMPO自旋捕集技术等三种方法分别在磷酸缓冲液、甲醇及二甲基亚砜(DMSO)中,测定超氧化物阴离子自由基(O₂^-·)。并与核黄素光照及邻苯三酚自氧化等二种已知产生O₂^-·系统相比较。结果表明:血卟啉衍生物光敏反应原初过程O₂^-·产率很低,水及DMSO等溶剂对OT的测定均有影响。

关键词: 血卟啉衍生物, 超氧化物自由基, 光敏化作用

Abstract: Three different methods-reduction of nitro-blue tetrezolium, reduction of cyto-chrome C and DMPO-Spin-trapping technique, were utilized to detect the formation of O₂^-· in phosphate-buffered solution, methyl alcohol and dimethyl sulfoxide (DMSO) respectively.As compared with riboflavine photosensitization and autooxidation of pyrogallol both systems for the generation of O₂^-·, the yield of O₂^-· formation by photosensitization reaction of HPD is quite low.It was also found that some soluents such as H₂O and DMSO, have an effect on the measurement of O₂^-·.

Key words: Hematoporphyrin derivative, Superoxide anion radical, Photosen-sitization

曹恩华, 辛淑敏. 血卟啉衍生物光照产生超氧化物阴离子自由基的一些观察[J]. 影像科学与光化学, 1991, 9(3): 191-197.

CAO EN-HUA, XIN SHU-MIN. SOME OBSERVATION ON THE SUPEROXIDE RADICAL FORMATION BY HEMATOPORPHYRIN DERIVATIVE PHOTOSENSITIZATION[J]. Imaging Science and Photochemistry, 1991, 9(3): 191-197.

血卟啉衍生物光照产生超氧化物阴离子自由基的一些观察

SOME OBSERVATION ON THE SUPEROXIDE RADICAL FORMATION BY HEMATOPORPHYRIN DERIVATIVE PHOTOSENSITIZATION

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