酸预处理对酶法骨明胶功能性质和分子质量分布的影响

doi:10.7517/j.issn.1674-0475.2012.02.102

影像科学与光化学 ›› 2012, Vol. 30 ›› Issue (2): 102-109.DOI: 10.7517/j.issn.1674-0475.2012.02.102

酸预处理对酶法骨明胶功能性质和分子质量分布的影响

张兵, 史京京, 王颖, 郭燕川, 陈丽娟

中国科学院理化技术研究所, 北京 100190

收稿日期:2011-10-09 修回日期:2011-12-30 出版日期:2012-03-15 发布日期:2012-03-15
通讯作者: 张兵(1976-),女,副研究员, 主要从事明胶和水解胶原蛋白的制备与应用研究,E-mail:zhangbing@mail.ipc.ac.cn.
基金资助:
中国科学院知识创新工程青年人才领域前沿项目(TYF0902).

Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process

ZHANG Bing, SHI Jing-jing, WANG Ying, GUO Yan-chuan, CHEN Li-juan

Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China

Received:2011-10-09 Revised:2011-12-30 Online:2012-03-15 Published:2012-03-15

摘要/Abstract

摘要： 本文以牛骨粉为原料,采用酶解法制备明胶,考察了骨粉预处理试剂——盐酸的浓度对明胶产品的冻力、粘度、分子量分布和等电点的影响.研究表明,随着骨粉预处理用的盐酸浓度的增大,明胶产率降低.骨粉经过盐酸预处理后,明胶产品的冻力大于300 bloom g,高于原料未经盐酸处理得到的明胶产品.与传统的碱法工艺明胶相比,酶法明胶具有高冻力、高等电点和相对较低的粘度等特点.采用聚丙烯酰胺凝胶电泳表征明胶的分子质量分布,酶法明胶不但具有传统碱法明胶具有的96 KDa、97 KDa、193 KDa和289 KDa组分,而且具有165 KDa和120 KDa组分.酶法明胶的等电点在碱性范围内,随着预处理用盐酸浓度的增大,明胶产品的等电点升高.骨料的预处理工艺对明胶的产率、冻力、粘度、等电点和分子质量分布有重要的影响,在明胶制备过程中,通过调控骨料预处理酸浓度可以调控明胶的技术指标.

关键词: 明胶, 酶解法, 酸预处理, 等电点, 分子量分布

Abstract: Gelatins were prepared from the bone pretreated using hydrochloride with various concentrations by protease-conditioned method in this article. The yields of protease-conditioned gelatin were decreased as the concentration of HCl was increased from 0 to 4.5% (mass fraction). The bloom values of protease-condition gelatin were all higher than 300 when the bovine bone were pretreated with HCl. Compared with commercial type B gelatin, protease-conditioned gelatin exhibited higher bloom strength, lower viscosity and higher IEP value. Compared with protein patterns of type B gelatin, different protein patterns of protease-conditioned gelatin were noticeable with the immersing of the new bands at the Mw of 165 KDa and 120 KDa. The IEPs of protease-conditioned gelatin were all in alkaline range which was related to the content of arginine. The results showed that the pretreatment process of bone was very important for protease-conditioned gelatin because the yield, viscosity and IEP were determined by the pretreatment condition.

Key words: gelatin, protease-conditioned, acid pretreatment, IEP, protein patterns

中图分类号:

张兵, 史京京, 王颖, 郭燕川, 陈丽娟. 酸预处理对酶法骨明胶功能性质和分子质量分布的影响[J]. 影像科学与光化学, 2012, 30(2): 102-109.

ZHANG Bing, SHI Jing-jing, WANG Ying, GUO Yan-chuan, CHEN Li-juan. Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process[J]. Imaging Science and Photochemistry, 2012, 30(2): 102-109.

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酸预处理对酶法骨明胶功能性质和分子质量分布的影响

Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process

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