苝二酰亚胺衍生物/三联吡啶铂配合物超分子在G-四链体检测中的应用

doi:10.7517/j.issn.1674-0475.2017.04.017

摘要/Abstract

摘要：

合成了3种三联吡啶铂（Ⅱ）配合物（Pt1、Pt2、Pt3）和两种苝二酰亚胺衍生物（PDI1、PDI2），构建了三联吡啶铂配合物/苝二酰亚胺衍生物超分子体系。所得超分子体系可作为"turn-on"型荧光传感器用于检测c-myc G-四链体。三联吡啶铂配合物可通过电子转移作用猝灭苝二酰亚胺的荧光，利用最小二乘法求得三联吡啶铂配合物与PDIs的结合常数为5.57×10⁴~5.28×10⁶ L/mol。加入G-四链体后，三联吡啶铂配合物/苝二酰亚胺超分子发生解离，苝二酰亚胺衍生物的荧光得到恢复。在Pt2/PDI2体系中加入1.5 μmol/L c-myc G-四链体可使其荧光增强63倍。在c-myc的浓度为25 nmol/L~1.0 μmol/L范围内，Pt2/PDI2超分子体系荧光增强（F/F₀）与c-myc的浓度呈良好的线性关系（R² = 0.995），检测限为1.37 nmol/L，表明Pt2/PDI2超分子体系可用于检测c-myc序列DNA。

关键词: 铂（Ⅱ）配合物, G-四链体, 苝二酰亚胺类化合物, 荧光传感器

Abstract:

Three terpyridine platinum complexes (Pt1-3) and two perylenediimide derivatives (PDI1-2) are synthesized, and employed to construct "turn-on" type fluorescent sensing ensembles for c-myc G-quadruplex. The fluorescence of perylenediimide derivatives can be efficiently quenched due to photoinduced electron transfer (PET) from platinum complex to perylenediimide. The ensembles exhibit moderate association constants in the range of 5.57×10⁴~5.28×10⁶ L/mol calculated by nonlinear least-squares curve-fitting method. Addition of G-quadruplex to the ensembles, the fluorescence of perylenediimide can be recovered due to the dissociation of the ensembles. For the Pt2/PDI2 system, the emission enhancement (F/F₀) is about 63-fold when the concentration of c-myc G-quadruplex is 1.5 μmol/L. The fluorescence enhancement shows an excellent linear relationship (R² = 0.995) with the concentration of c-myc in the range of 25 nmol/L~1.0 μmol/L, and the limit of detection is 1.37 nmol/L, indicating that the Pt2/PDI2 ensemble is sensitive to c-myc.

Key words: platinum (Ⅱ) complex, G-quadruplex, perylenediimides, fluorescence sensor

高云燕, 蔡温姣, 欧植泽, 马拖拖, 王子继, 许墨横. 苝二酰亚胺衍生物/三联吡啶铂配合物超分子在G-四链体检测中的应用[J]. 影像科学与光化学, 2017, 35(4): 552-562.

GAO Yunyan, CAI Wenjiao, OU Zhize, MA Tuotuo, WANG Ziji, XU Moheng. Detection of G-quadruplex by Supramolecular Systems Assembled by Perylenediimide Derivatives and Terpyridineplatinum(Ⅱ) Complexes[J]. Imaging Science and Photochemistry, 2017, 35(4): 552-562.

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