影像科学与光化学 ›› 2017, Vol. 35 ›› Issue (6): 780-787.DOI: 10.7517/j.issn.1674-0475.2017.05.004

• 论文 • 上一篇    下一篇

双靶向CdTe量子点的制备及其在细胞标记中的应用

杨冬芝1,2, 王文文2, 杜岩1,2, 纪海侠1,2   

  1. 1. 徐州医科大学 药物分析教研室, 江苏 徐州 221004;
    2. 徐州医科大学 新药研究与临床药学重点实验室, 江苏 徐州 221004
  • 收稿日期:2016-12-09 修回日期:2017-01-10 出版日期:2017-11-15 发布日期:2017-11-15
  • 通讯作者: 杨冬芝
  • 基金资助:

    国家自然科学基金(81201696)、江苏省自然科学基金(BK20161173)、国家大学生创新创业训练计划(20131031300)和江苏省高等学校大学生实践创新训练计划项目(201310313004Z)

The Preparation of Dual Targeting CdTe Quantum Dots and Its Application in Cells Labeling

YANG Dongzhi1,2, WANG Wenwen2, DU Yan1,2, JI Haixia1,2   

  1. 1. Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China;
    2. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China
  • Received:2016-12-09 Revised:2017-01-10 Online:2017-11-15 Published:2017-11-15

摘要:

以巯基乙酸和巯基乙酰肼为稳定剂,制备了酸度敏感型CdTe量子点。经与抗体链接,该量子点具备酸度敏感、免疫识别双重靶向功能。经荧光光谱分析、透射电镜图像及细胞免疫成像证明,抗体已成功链接于量子点表面,且该量子点具有酸度敏感及抗体识别的双重靶向功能,可以实现对肿瘤细胞的特异性标记。

关键词: CdTe量子点, 酸度敏感性, 免疫靶向

Abstract:

The pH sensitive CdTe quantum dots (QDs) were prepared with thioglycolic acid (TGA) and mercapto-acetohydrazide (TGH) as the stabilizers. After being conjugated with antibody, the prepared CdTe QDs has both functions of pH sensitivity and immune recognition. The results of fluorescence luminescence (FL), transmission electron microscopy (TEM) and cells labeling imaging showed that antibody was successfully conjugated on the surface of CdTe QDs. The prepared dual targeting QDs can be used to identify the acidic interstitial extracellular environment and label tumor cells specifically.

Key words: CdTe quantum dots, pH sensitivity, immune recognition