CdTe量子点与蛋白质相互作用的荧光猝灭效应

doi:10.7517/j.issn.1674-0475.2014.02.181

影像科学与光化学 ›› 2014, Vol. 32 ›› Issue (2): 181-190.DOI: 10.7517/j.issn.1674-0475.2014.02.181

CdTe量子点与蛋白质相互作用的荧光猝灭效应

焦勇¹, 李荣霞¹, 安文汀², 李世琴¹, 赵俊红¹

1. 山西大学分子科学研究所化学生物学与分子工程教育部重点实验室, 山西太原 030006;
2. 山西大学化学化工学院, 山西太原 030006

收稿日期:2013-10-10 修回日期:2014-01-22 出版日期:2014-03-15 发布日期:2014-03-15
通讯作者: 焦勇, 安文汀
基金资助:
国家自然科学基金项目（20971081）及山西省自然科学基金项目（2010011010）资助

Fluorescence Quenching Effect of the Interactions between CdTe Quantum Dots and Proteins

JIAO Yong¹, LI Rongxia¹, AN Wenting², LI Shiqin¹, ZHAO Junhong¹

1. Key Laboratory of Ministration of Education for Chemical biology & Molecular Engineering, Institute of Molecular Science, Shanxi University, Taiyuan 030006, Shanxi, P. R. China;
2. School of Chemistry & Chemical Engineering, Shanxi University, Taiyuan 030006, Shanxi, P. R. China

Received:2013-10-10 Revised:2014-01-22 Online:2014-03-15 Published:2014-03-15

摘要/Abstract

摘要：

本文在水热法合成水溶性CdTe及核壳结构CdTe/CdS量子点的基础上，分别研究了细胞色素c对CdTe量子点及CdTe/CdS核壳量子点荧光的猝灭效应和CdTe量子点对牛血清白蛋白荧光的猝灭效应，并阐述了猝灭机理。结果显示，细胞色素c对CdTe量子点的荧光猝灭效应具有一定的粒径依赖性，粒径越小，猝灭效应越强；细胞色素c对CdTe/CdS核壳量子点的猝灭效应比对CdTe量子点的更强，揭示了受激电子的表面传递机理。CdTe量子点通过松散牛血清白蛋白的螺旋结构而猝灭其荧光。

关键词: CdTe量子点, CdTe/CdS核壳量子点, 细胞色素c, 牛血清白蛋白, 荧光猝灭效应, 荧光猝灭机理

Abstract:

On the basis of synthesizing of water-soluble CdTe and CdTe/CdS core-shell quantum dots (QDs) by hydrothermal methods, this work studied the quenching effects of cytochrome c (Cyt c) to the fluorescence of CdTe QDs and CdTe/CdS core-shell QDs,as well as CdTe QDs to the fluorescence of bovine serum albumin (BSA), respectively, and clarified the fluorescence quenching mechanisms. The results showed that the fluorescence quenching effects of Cyt c to CdTe QDs were dependent on the QDs size to a certain degree, and the smaller the sizes of CdTe QDs, the stronger the quenching effects. In addition, the quenching effects of Cyt c to CdTe/CdS core-shell QDs are stronger than that to CdTe QDs, indicating the surface transfer mechanism of excited electron. The fluorescence quenching effects of CdTe QDs to bovine serum albumin (BSA) were through the way of relaxing the helix structures of BSA.

Key words: CdTe quantum dots, CdTe/CdS core-shell quantum dots, cytochrome c, bovine serum albumin, fluorescence quenching effect, fluorescence quenching mechanism

焦勇, 李荣霞, 安文汀, 李世琴, 赵俊红. CdTe量子点与蛋白质相互作用的荧光猝灭效应[J]. 影像科学与光化学, 2014, 32(2): 181-190.

JIAO Yong, LI Rongxia, AN Wenting, LI Shiqin, ZHAO Junhong. Fluorescence Quenching Effect of the Interactions between CdTe Quantum Dots and Proteins[J]. Imaging Science and Photochemistry, 2014, 32(2): 181-190.

参考文献

[1] Chan W C, Nie S. Quantum dot bioconjugates for ultrasensitive nonisotopic detection[J]. Science, 1998, 281(5385): 2016-2018.
[2] Bruchez M, Moronne M, Gin P, et al. Semiconductor nanocrystals as fluorescent biological labels[J]. Science, 1998, 281(5385): 2013-2016.
[3] Michalet X, Pinaud F F, Bentolila L A, et al. Quantum dots for live cells, in vivo imaging and diagnostics[J]. Science, 2005, 307(5709): 538-544.
[4] Zhang C Y, Yeh H C, Kuroki M T, et al. Single-quantum-dot-based DNA nanosensor[J]. Nature Materials, 2005, 4(11): 826-831.
[5] Klostranec J M, Chan W C. Quantum dots in biological and biomedical research: recent progress and present challenges[J]. Advanced Materials, 2006, 18(15): 1953-1964.
[6] Somers R C, Bawendi M G, Nocera D G. CdSe nanocrystal based chem-/bio-sensors[J]. Chemical Society Review, 2007, 36(4):579-591.
[7] Zhou D J, Ying L M, Hong X, et al. A compact functional quantum dot-DNA conjugate: preparation, hybridization, and specific label-free DNA detection[J]. Langmuir, 2008, 24(5): 1659-1664.
[8] Medintz I L, Mattoussi H. Quantum dot-based resonance energy transfer and its growing application in biology[J]. Physical Chemistry Chemical Physics, 2009, 11(1):17-45.
[9] Prasuhn D E, Blanco-Canosa J B, Vora G J, et al. Combining chemoselective ligation with polyhistidine-driven self-assembly for the modular display of biomolecules on quantum dots[J]. ACS Nano, 2010, 4(1):267-278.
[10] Jhonsi M A, Kathiravan A, Renganathan R. Spectroscopic studies on the interaction of colloidal capped CdS nanoparticles with bovine serum albumin[J]. Colloid and Surface B:Biointerfaces, 2009, 72(2): 167-172.
[11] Xiao Q, Huang S, Qi Z D, et al. Conformation, thermodynamics and stoichiometry of HSA adsorbed to colloidal CdSe/ZnS quantum dots[J]. Biochimica et Biophysica Acta -Proteins and Proteomics, 2008, 1784(7-8): 1020-1027.
[12] Shao L W, Dong C Q, Ren J C, et al. Using capillary electrophoresis mobility shift assay to study the interaction of CdTe quantum dots with bovine serum albumin[J]. Chinese Chemical Letters, 2008, 19(6): 707-710.
[13] Shao L W, Dong C Q, Ren J C, et al. Studies on interaction of CdTe quantum dots with bovine serum albumin using fluorescence correlation spectroscopy[J]. Journal of Fluorescence, 2009, 19(1): 15l-157.
[14] Ma Y, Bai H, Yang X, et al. A sensitive method for the detection of proteins by high-efficiency fluorescence quenching[J]. The Analyst, 2005, 130(3): 283-285.
[15] Larsen R W, Omdal D, Jasuja H R, et al. Conformational modulation of electron transfer within electrostatic porphyrin cytochrome c complexes[J]. Journal of Physical Chemistry B, 1997, 101(40): 8012-8020.
[16] Croney J C, Helms M K, Jameson D M, et al. Temperature dependence of photoinduced electron transfer within self-assembled uroporphyrin-cytochrome c complexes[J]. Journal of Physical Chemistry B, 2000, 104(5): 973-977.
[17] Clark-Ferris K K, Fischer J. Topographical mimicry of the enzyme binding domain of cytochrome c[J]. Journal of American Chemical Society, 1985, 107(17): 5007-5008.
[18] Cesar A T L, Sivia M B C, David P, et al. Electron-transfer kinetics in sulfonated aluminum phthalocyanines/cytochrome c complexes[J]. Journal of Physical Chemistry B, 2004, 108(22): 7506-7514.
[19] Yamasaki K, Maruyama T, Kragh-Hansen U, et al. Characterization of site on human serum albumin: concept about the structure of a drug binding site[J]. Biochimica et Biophysica Acta, 1996, 1295(2):147-157.
[20] Dockal M, Carter D C, Ruker F. Conformational transitions of the three recombinant domains of human serum albumin depending on pH[J]. Journal of Biological Chemistry, 2000, 275(5): 3042-3050.
[21] Hu Y, Liu Y, Zhao R, et al. Spectroscopic studies on the interaction between methylene blue and bovine serum albumin[J]. Journal of Photochemistry and Photobiology A: Chemistry, 2006, 179(3): 324-329.
[22] Zhou B, Qi Z D, Xiao Q, et al. Interaction of loratadine with serum albumins studied by fluorescence quenching method[J]. Journal of Biochemical and Biophysical Methods, 2007, 70(5): 743-747.
[23] Zhao D, He Z, Chan W H, et al. Synthesis and characterization of high-quality water-soluble near-infrared-emitting CdTe/CdS quantum dots capped by N-Acetyl-L-cysteine via hydrothermal method[J]. Journal of Physical Chemistry C, 2009, 113(4): 1293-1300.
[24] Yu W W, Qu L, Guo W, et al. Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals[J]. Chemical Materials, 2003, 15(14): 2854-2860.
[25] Crosby G A, Demas J N. Measurement of photoluminescence quantum yields[J]. Journal of Physical Chemistry, 1971, 75(8): 991-1024.
[26] 吴晓春, 陈文驹. 半导体量子点电子结构理论研究的进展[J]. 物理, 1995, 24(4): 218-223. Wu X C, Chen W J. Progress of the electronic structure theory of semiconductor quantum dots[J]. Physics, 1995, 24(4): 218-223.
[27] Lakowicz J R. Principles of Fluorescence Spectroscopy[M]. Second Edition. New York: Springer, 1999. 248-249.

CdTe量子点与蛋白质相互作用的荧光猝灭效应

Fluorescence Quenching Effect of the Interactions between CdTe Quantum Dots and Proteins

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价

[1]	杨冬芝, 王文文, 杜岩, 纪海侠. 双靶向CdTe量子点的制备及其在细胞标记中的应用[J]. 影像科学与光化学, 2017, 35(6): 780-787.
[2]	覃光炯, 高云华. 同步荧光法测定大鼠血浆中牛血清白蛋白的研究[J]. 影像科学与光化学, 2010, 28(5): 354-361.
[3]	许世超, 姚翠翠, 张纪梅, 代昭, 孙波, 郑帼, 韩青, 胡斐, 周鸿明. 表面修饰CdTe量子点的合成及其光学特性[J]. 影像科学与光化学, 2009, 27(3): 161-167.
[4]	杨坤, 陈欢, 赵文超, 王建涛, 王辉, 张晓宏. 硅纳米线阵列电极作为细胞色素c传感器的研究[J]. 影像科学与光化学, 2009, 27(2): 87-94.
[5]	白凤莲, 贾秀娟, 李玉良, 莫亦明, 朱道本 . 3,4,9,10-四甲基苝荧光性质的研究[J]. 影像科学与光化学, 1994, 12(2): 182-186.