同步荧光法测定大鼠血浆中牛血清白蛋白的研究

doi:10.7517/j.issn.1674-0475.2010.05.354

影像科学与光化学 ›› 2010, Vol. 28 ›› Issue (5): 354-361.DOI: 10.7517/j.issn.1674-0475.2010.05.354

同步荧光法测定大鼠血浆中牛血清白蛋白的研究

覃光炯^1,2, 高云华¹

1. 中国科学院, 理化技术研究所, 光化学转换与功能材料重点实验室, 北京, 100190;
2. 中国科学院, 研究生院, 北京, 100049

收稿日期:2010-05-11 修回日期:2010-05-25 出版日期:2010-09-23 发布日期:2010-09-23
通讯作者: 高云华,E-mail:yhgao@mail.ipc.ac.cn.

Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry

QIN Guang-jiong^1,2, GAO Yun-hua¹

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2010-05-11 Revised:2010-05-25 Online:2010-09-23 Published:2010-09-23

摘要/Abstract

摘要： 以异硫氰酸荧光素标记的牛血清白蛋白为模型药物,采用同步荧光法测定了大鼠尾静脉注射模型药物后血浆中药物浓度随时间的变化.系统研究了样品处理、测试条件等一系列因素对测试结果的影响,建立了一种简单有效的测定生物体内的微量外源性蛋白质的方法,并与酶联免疫吸附法测定结果进行了比较,二者的测试结果具有良好的一致性.

关键词: 同步荧光法, 牛血清白蛋白, 血药浓度, 酶联免疫吸附法

Abstract: With fluorescein isothiocyanate labeled bovine serum albumin(FITC-BSA) as a model drug,the concentration of FITC-BSA in rat plasma was assayed after tail vein injection by utilizing synchronous fluorescence spectrometry.The impacts of sample pretreatment,measurement parameters were investigated.A simple and effective determination method for exogenous protein in vivo was established by utilizing synchronous spectrometry,whose results match with results by standard enzyme-linked immunosorbent assay(ELISA) very well.

Key words: synchronous fluorescence, bovine serum albumin, enzyme-linked immunosorbent assay

中图分类号:

O657

覃光炯, 高云华. 同步荧光法测定大鼠血浆中牛血清白蛋白的研究[J]. 影像科学与光化学, 2010, 28(5): 354-361.

QIN Guang-jiong, GAO Yun-hua. Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry[J]. Imaging Science and Photochemistry, 2010, 28(5): 354-361.

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同步荧光法测定大鼠血浆中牛血清白蛋白的研究

Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry

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