硅纳米线阵列电极作为细胞色素c传感器的研究

doi:10.7517/j.issn.1674-0475.2009.02.87

影像科学与光化学 ›› 2009, Vol. 27 ›› Issue (2): 87-94.DOI: 10.7517/j.issn.1674-0475.2009.02.87

硅纳米线阵列电极作为细胞色素c传感器的研究

杨坤^1,2, 陈欢^1,2, 赵文超^1,2, 王建涛^1,2, 王辉¹, 张晓宏¹

1. 中国科学院, 理化技术研究所, 纳米有机光电子实验室, 北京, 100190;
2. 中国科学院, 研究生院, 北京, 100039

收稿日期:2008-06-12 修回日期:2008-07-02 出版日期:2009-03-23 发布日期:2009-03-23
基金资助:
国家重大科学研究项目(973,2006CB933000);国家高技术研究发展项目(863,2006AA03Z302)

Silicon Nanowire Arrays as Electrochemical Biosensors for Cytochrome c

YANG Kun^1,2, CHEN Huan^1,2, ZHAO Wen-chao^1,2, WANG Jian-tao^1,2, WANG Hui¹, ZHANG Xiao-hong¹

1. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. Graduate University of the Chinese Academy of Sciences, Beijing 100039, P.R. China

Received:2008-06-12 Revised:2008-07-02 Online:2009-03-23 Published:2009-03-23

摘要/Abstract

摘要： 利用化学刻蚀法由p型硅片制备了硅纳米线阵列,经过表面去氧化层处理后,制备了检测蛋白质细胞色素c的电化学传感器.实验表明,硅纳米线阵列电极对细胞色素c有良好的电化学响应,并且在低浓度条件下具备线性响应的特点.根据与未经表面处理的硅纳米线阵列电极的实验结果相对比,提出了细胞色素c所具备的羧基末端与硅纳米线阵列电极表面的Si-H相互作用从而改善传感性能的检测机理.

关键词: 硅纳米线阵列, 电化学传感器, 细胞色素c

Abstract: The direct electrochemical biosensor for cytochrome c based on the silicon nanowire arrays was fabricated.The silicon nanowire arrays were prepared from the etched silicon wafer,which were then treated with aqua regia and HF to remove the cladding silicon oxide sheath.The modified silicon nanowire arrays were further used as the electrode for fabricating the direct electrochemical biosensors for the detection of cytochrome c,which exhibited linear response to cytochrome c at low concentration and high sensitivity of 2.75 μA·mmol^-1·L due to the vast surface-to-bulk ratio of the silicon nanowire arrays and the interaction between the carboxyl group of cytochrome and Si-H group provided by the surface of the modified silicon nanowire arrays.

Key words: silicon nanowire arrays, electrochemical biosensors, cytochrome c

中图分类号:

杨坤, 陈欢, 赵文超, 王建涛, 王辉, 张晓宏. 硅纳米线阵列电极作为细胞色素c传感器的研究[J]. 影像科学与光化学, 2009, 27(2): 87-94.

YANG Kun, CHEN Huan, ZHAO Wen-chao, WANG Jian-tao, WANG Hui, ZHANG Xiao-hong. Silicon Nanowire Arrays as Electrochemical Biosensors for Cytochrome c[J]. Imaging Science and Photochemistry, 2009, 27(2): 87-94.

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硅纳米线阵列电极作为细胞色素c传感器的研究

Silicon Nanowire Arrays as Electrochemical Biosensors for Cytochrome c

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