影像科学与光化学 ›› 1998, Vol. 16 ›› Issue (3): 258-263.DOI: 10.7517/j.issn.1674-0475.1998.03.258

• 研究简报 • 上一篇    下一篇

胰蛋白酶降解骨胶原与明胶组份含量之间的关系

陈丽娟, 王颖, 彭必先   

  1. 中国科学院感光化学研究所, 北京 100101
  • 收稿日期:1997-03-27 修回日期:1998-01-16 出版日期:1998-08-20 发布日期:1998-08-20
  • 通讯作者: 陈丽娟

THE RELATIONSHIP BETWEEN THE TRYPSIN DIGESTION OF BONE COLLAGEN AND THE COMPONENT CONTENT OF GELATIN

CHEN Lijuan, WANG Ying, PENG Bixian   

  1. Institute of Photographic Chemistry, The Chinese Academy of Sciences, Beijing 100101, P. R. China
  • Received:1997-03-27 Revised:1998-01-16 Online:1998-08-20 Published:1998-08-20

摘要: 国内外的传统碱法制备明胶,无论是工艺还是最终产品质量,都已经达到了很高的水平,但仍存在很大的缺点,如浸灰周期长(一般1~2个月),生产效率低等。酶是一种有催化活性的蛋白质,能以非常低的浓度来增加正反应或逆反应的速度而不消耗自身。

关键词: 明胶, 胶原, 胰蛋白酶,

Abstract: It is indicated in present study that the bone material can be digested by trypsin. An attempt was made to correlate the relative content of different conformation components with the concentration of trypsin. It is shown that the component (such as α 1) content was not in direct proportion to the trypsin concentration and a maxium was found. The experiment showed that the migration position of the components (in particular,α,β and γ) of enzyme-processed gelatin is completely coincided with that of gelatin produced by alkaline-processed method at industrial scale in electrophoresis scanning pattern. The paper also discussed the feature of molecular structure of enzyme-processed gelatin and the possible mechanism.

Key words: gelatin, collagen, trypsin, enzyme