DECAY KINETICS OF M-STATE IN BACTERIORHODOPSIN- POLY(VINYL ALCOHOL) FILMS

doi:10.7517/j.issn.1674-0475.2001.02.96

Abstract

Abstract: The influence of increase of pH and dehydration time on the decay kinetics of M-state in bacteriorhodopsin (bR)-poly(vinyl alcohol) (PVA) films was studied by UV-vis spectrophotometry and flash photolysis under room conditions. Flash photolysis study showed that the M-state lifetime was prolonged rather obviously by increasing of pH and dehydration time. The slowest decay of M-state was observed in bR-PVA film prepared at pH 10 and dehydration time of 72 h and UV-vis spectrophotometry indicated that the M-state could sustain over 15 min, which represented a four order of magnitude improvement over that of native bR. In addition, the mechanism for the prolongation of M-state in bR-PVA films was discussed in this paper.

Key words: bacteriorhodopsin, photocycle, M-state, photochromism

CLC Number:

O644

LI Mei-ling, LI Bao-fang, JIANG Long. DECAY KINETICS OF M-STATE IN BACTERIORHODOPSIN- POLY(VINYL ALCOHOL) FILMS[J]. Imaging Science and Photochemistry, 2001, 19(2): 96-101.

References

[1] Oesterhelt D, Stockenius W. Rhodopsin-like protein from the purple membrane of Halobacterium halobium[J]. Nature New Biol., 1971,233:149-152.
[2] Thoma R, Hampp N, Brauchle C. Bacteriorhodopsin films as spatial light modulators for non-linear filtering[J]. Opt. Lett., 1991,16:165-169.
[3] Hampp N, Brauchle C, Oesterhelt D. Bacteriorhodopsin and its genetic variants as media for dynamic holographic information processing[J]. IEE Conf. Publ., 1991,342:35-39.
[4] Keszthelyi L, Ormos P, Varo G. Fast components of the electric response signal of bacteriorhodopsin protein[J]. Acta Phys. Acad. Sci. Hung., 1982,53:143-157.
[5] Korenstein R, Hess B. Hydration effects on the photocycle of bacteriorhodopsin in thin layers of membrane[J]. Nature, 1977,270:184-186.
[6] Oesterhelt D, Stoeckenius W. Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane[J]. Methods Enzymol., 1974,31:667-678.
[7] Hildebrant P, Stockburger M. Role of water in bacteriorhodopsin's chromophore: Resonance raman study[J]. Biochemistry, 1984,23:5539-5548.
[8] Ormos P, Hristova S, Keszthelyi L. The effect of pH on proton transport by bacteriorhodopsin[J]. Biochimi. Biophys. Acta, 1985, 809:181-186.
[9] Gross R, Izgi K C, Birge R R. Holographic thin films, spatial light modulators and optical associative memories based on bacteriorhodopsin[J]. SPIE, 1992,1662:186-196.
[10] Keszthelyi L, Ormos P, Varo G. Fast components of the electric response signal of bacteriorhodopsin protein[J]. Acta Phys. Acad. Sic. Hung., 1982,53:143-157.
[11] Zaccai G. Structure and hydration of purple membranes in different conditions[J]. J. Mol. Biol., 1987,194:569-572.
[12] Eisfield W, Pusch C, Stockburger M. Resonance raman and optical transitent studies on the light-induced proton pump pf bacteriorhodopsin reveal parallel photocycles[J]. Biochemistry, 1993,32:7196-7215.

[1]	CHEN Yidan, WANG Jiameng, WANG Guang. Development of Naphthopyran-based Photochromic Fluorescence Switching [J]. Imaging Science and Photochemistry, 2017, 35(3): 233-245.
[2]	WANG Renjie, LIU Gang, PU Shouzhi. Research Progress of Optical Controlling Diarylethene Chemical Sensors [J]. Imaging Science and Photochemistry, 2017, 35(3): 246-264.
[3]	WANG Xinrui, TANG Liping, LI Yong, CHENG Huimin, YU Tingting, YAN Dongpeng. The Photosensitization Based on Host-guest Interactions in a Layered Double Hydroxide [J]. Imaging Science and Photochemistry, 2017, 35(3): 307-315.
[4]	LU Guanglei, LIU Keyin, MENG Luyan, YI Tao. Development and Application of Diarylethene Based Fluorescent Switch [J]. Imaging Science and Photochemistry, 2014, 32(1): 28-42.
[5]	GONG Wenliang, XIONG Zujin, ZHU Mingqiang. Current Progress in Molecular Photoswitches Based on Hexaarylbiimidazole： Molecular Design and Synthesis, Properties and Application [J]. Imaging Science and Photochemistry, 2014, 32(1): 43-59.
[6]	MENG Qing-hua, FANG Yong-zeng, WANG Zhen, HUANG De-yin. The Study of Extended π Conjugation Systems in Photochromic Naphthopyran Compounds [J]. Imaging Science and Photochemistry, 2012, 30(2): 150-158.
[7]	LI Zhi-bin, LI Ling, ZHANG Zhen-lin, WANG Yue-chuan. Preparation and Photochromic Properties of Viologen/Polymer Film Blended with Copper Chloride [J]. Imaging Science and Photochemistry, 2010, 28(1): 29-36.
[8]	HU Shi-rong, FANG Yue, HUANG Cheng-jin, ZHANG Yu-ping, DU Jin-zhen, CHEN Bo-yong. Synthesis and Photochromism of the 2-Aryl-1,8-Phthaloylnaphthalene [J]. Imaging Science and Photochemistry, 2009, 27(2): 135-140.
[9]	GU Ling-yan, ZHANG Qing, SHENG Qiao-rong, XUE Min-zhao, LIU Yan-gang. Synthesis of Spiropyran with BOC Protective Function and Research of Photochromism [J]. Imaging Science and Photochemistry, 2008, 26(2): 81-87.
[10]	SHI Ming, NIU Li-hong, TONG Qing-xiao, TANG Ying-wu, ZHANG Fu-shi. The Application of Photochromic Diarylethene Mask Layer in the Near-Field Optical Storage [J]. Imaging Science and Photochemistry, 2007, 25(3): 204-208.
[11]	HU Shi-rong, ZHAO Feng, FANG Yue, CAI Zu-chang. Study on the Synthesis and Photochromic Mechanism of the Phenoxyanthraquinone [J]. Imaging Science and Photochemistry, 2006, 24(4): 306-311.
[12]	LI Gui-zhen, PU Shou-zhi, XU Jing-kun, SHEN Liang, XIAO Qiang, CHEN Bing, ZHAO Ji-quan. Synthesis of New Types Photochromic Dithienylethenes and Investigation on their Properties [J]. Imaging Science and Photochemistry, 2005, 23(3): 167-174.
[13]	CHU Jin-fang, TANG Ji-an. PURIFICATION OF PHOTOSENSITIVE PROTEIN FROM HALOPHILE [J]. Imaging Science and Photochemistry, 2002, 20(5): 391-398.
[14]	HE Wei, ZHOU Jinwei, SUI Qiang, LI Zhongjiea, WANG Yanqiao. SYNTHESIS, CHARACTERIZATION, ADN INVESTIGATION OF PHOTOCHROMIC PROPERTIES OF TWO NOVEL CATIONIC INDOLINOSPIROPYRANS [J]. Imaging Science and Photochemistry, 1999, 17(3): 264-269.
[15]	YUAN Linshan, LI Baofang, JIANG Long. A STUDY OF INFLUENCE OF POLYPYRROLE FILM TO THE PHOTOELECTRIC PROPERTIES OF LANGMUIR- BLODGETT FILM CONTAINING BACTERIORHODOPSIN [J]. Imaging Science and Photochemistry, 1998, 16(4): 300-302.