Imaging Science and Photochemistry ›› 2007, Vol. 25 ›› Issue (5): 321-326.DOI: 10.7517/j.issn.1674-0475.2007.05.321

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Two-Photon Excited Fluorescent Imaging for Cu2+ in Living Cells

GAO Yan-hong1, YU Meng-xiao2, LI Fu-you2, LI Ding-guo1, YI Tao2, LU Han-ming1, HUANG Chun-hui2   

  1. 1. Department of Digestive Disease & Geriatrics, Xinhua Hospital, Medicine School of Shanghai Jiaotong University, Shanghai 200092, P. R. China;
    2. Department of Chemistry, and Laboratory of Advanced Materials, Fudan University, Shanghai 200433, P. R. China
  • Received:2007-05-17 Revised:2007-06-12 Online:2007-09-23 Published:2007-09-23

Abstract: Based on the spirolactam (nonfluorescent) to ring-open amide (fluorescent) equilibrium, a dye FD2 was demonstrated as a highly selective sensor for Cu2+ over other metal ions. This probe is the first Cu2+ chemosensor with turn-on (85-fold) two-photon excited fluorescence.Moreover, two-photon fluorescent microscopy experiments further establish that FD2 can be used for sensing Cu2+ within living cells.

Key words: fluorescent bioimaging, Cu2+, two-photon excited fluorescence

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