Glucose Biosensor Based on the Gold Nanorod/Chitosan Composite Film as Immobilization Matrix

doi:10.7517/j.issn.1674-0475.2008.04.271

Abstract

Abstract: Au nanorod /chitosan have good biocompatibility,and are suitable for enzyme immobilization.In this paper,Au nanorod /chitosan composite matrix was developed to fabricate the glucose biosensor.The fabrication of electrode is simple and easily operated.The enzyme electrode was characterized by electrochemistry impedance spectrum and cyclic voltammetry,and the equivalent circuit was given.The experimental results indicated that the Au nanorod/chitosan composite membrane could increase the electronic transfer and response current of electrode.The linear range was 2.78×10^-5 to 2.22×10^-3 mol/L with a detection limit of 5.0×10-6 mol/L at 3 σ and a sensitivity of 7.819 μA·cm^-2(mmol/L)^-1,the apparent Michaelis-Menten constant(K_m) is 10.387 mmol/L.The current response maintained 80% of original response after the electrode was repeatedly used 50 times.Further,it was found that 35 ℃ and 6.8 of pH are the set of optimal for the electrode measurement.

Key words: Au nanorod, chitosan, glucose oxidase, biosensor, electrochemistry impedance spectrum

CLC Number:

DU Ai-ming, REN Xiang-ling, TANG Fang-qiong, ZHANG Lin, LIU Feng-ling. Glucose Biosensor Based on the Gold Nanorod/Chitosan Composite Film as Immobilization Matrix[J]. Imaging Science and Photochemistry, 2008, 26(4): 271-279.

References

[1] Clark L C,Lyons C.Electrode systems for continuous.monitoring in cardiovascular surgery[J].Ann.NY Aead.Sci.,1962,102:29-45.
[2] Updike S J,Hicks G P.The enzyme electrode[J].Nature,1967,214:986-988.
[3] 任湘菱,唐芳琼.金属纳米颗粒的制备及其在酶生物传感器中的应用研究[J].感光科学与光化学(影像科学与光化学),2004,22(5):398-398.Ren X L,Tang F Q.Preparation of mental nanoparticles andtheir applications to glucose biosensor[J].Photographic Science and Photochemstry(Imaging Science and Photochemistry),2004,22(5):398-398.
[4] Huang Y X,Zhang WJ,Xiao H,et al.An electrochemical investigation of glucose oxidase at a CdS nanoparticles modified electrode[J].Biosens.Bioelectron.,2005,21(5):817-821.
[5] Xie Y B,Zhou L M,Huang HT.Bioelectrocatalytic application of titania nanotube array for molecule detection[J].Biosens.Bioelectron.,2007,22(12):2812-2818.
[6] 唐芳琼,韦正,陈东,等.亲水金和憎水二氧化硅纳米颗粒对葡萄糖生物传感器响应灵敏度的增强作用[J].高等学校化学学报,2000,21(1):91-94.Tang F Q,Wei Z,Chen D,et al.Enhancement effect of hydrophilic Au and hydrophobic Si O2nanoparticles on glu-cose biosensor sensitivity[J].Chem.J.Chinese Universitie,2000,21(1):91-94.
[7] Ren X L,Meng X W,Tang F Q.Preparation of Ag-Au nanoparticle andits applicationto glucose biosensor[J].Sen-sor Actuat B-Chem.,2005,110(92):358-363.
[8] Ren X L,Meng X W,Chen D,et al.Usingsilver nanoparticleto enhance current response of biosensor[J].Biosens.Bioelectron.,2005,21(3):433-437.
[9] 孙莹莹,赵爽,杨微微,等.基于层-层自反应的葡萄糖氧化酶有序多层膜电极[J].高等学校化学学报,2006,27(5):839-844.Sun Y Y,Zhao S,Yang P P,et al.Ordered multiayer fil melectrode containing glucose oxidase based on layer-by-layer self-reaction[J].Chem.J.Chinese Universities,2006,27(5):839-844.
[10] Koudelka-Hep M,Wai P D.Microelectrode sensorsfor biomedical and environmental applications[J].Electrochi m.Acta,2000,45(15-16):2437-2441.
[11] Takhistiv P.Electrochemical synthesis and i mpedance characterization of nano-patterned biosensor substrate[J].Biosens.Bioelectron.,2004,19(11):1445-1456.
[12] Wen D,Liu Y,Yang G C,et al.Electrochemistry of glucose oxidasei mmobilized onthe carbon nanotube wrapped by polyelectrolyte[J].Electrochi mica Acta,2007,52(16):5312-5317.
[13] Delvaux M,Walcarius A,Demoustier-Champange S.Bienzyme HRP-GOx-modified gold nanoelectrodes for the sen-sitive amperometric detection of glucose at lowoverpotentials[J].Biosens.Bioelectron.,2005,20(8):1587-1594.
[14] Crespilho F N,Zucolotto V,Brett C MA,et al.Enhanced charge transport andincorporation of redox mediatorsin layer-by-layer fil ms containing PAMAM-Encapsulated gold nanoparticles[J].J.Phys.Chem.B,2006,110(35):17478-17483.
[15] Wu B Y,Hou S H,Yin F,et al.Amperometric glucose biosensor based on layer-by-layer assembly of multilayer fil ms composed of chitosan,gold nanoparticles and glucose oxidase modified Pt electrode[J].Biosens.Bioelectron,2007,22(6):838-844.
[16] 张立德,张玉刚.一维纳米材料中的新效应和新功能[J].功能材料,2004,35(增刊):50-53.Zhang L D,Zhang Y G.Neweffects and newfunctions of one-di mensional nanomaterials[J].Journal of Functional Materials.,2004,35:50-53.
[17] Gou L F,Murphy CJ.Fine-tuning the shape of gold nanorods[J].Chem.Mater.,2005,17(14):3668-3672.
[18] Sali mi A,Sharifi E,Noorbakhsh A,et al.I mmobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles:Direct electron transfer and electrocatalytic activity[J].Biosens.Bioelectron,2007,22(12):3146-3153.
[19] Murthy AS N,Sharma J.Glucose oxidase bound to self-assembled monolayers of bis(4-pyridyl)disulfide at a gold electrode:Amperometric determination of glucose[J].Anal.Chi m.Acta,1998,363(2-3):215-220.