Study on the Protein Diffusion Behavior in the Poly(Acrylamide-co-Acrylic Acid) Copolymer by FRAP Technique

doi:10.7517/j.issn.1674-0475.2011.05.336

Imaging Science and Photochemistry ›› 2011, Vol. 29 ›› Issue (5): 336-343.DOI: 10.7517/j.issn.1674-0475.2011.05.336

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Study on the Protein Diffusion Behavior in the Poly(Acrylamide-co-Acrylic Acid) Copolymer by FRAP Technique

CUI Jin-lei^1,2, ZHOU Wen-wen^1,2, QIU Yu-qin¹, GAO Yun-hua¹

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2011-04-14 Revised:2011-04-28 Online:2011-09-23 Published:2011-09-23

Abstract

Abstract: The drug release behavior from the carrier is bound up with the carrier’s structure.In this paper,a series of poly(acrylamide-co-acrylic acid) hydrogels with different acrylamide/acrylic acid ratios have been successfully synthesized by aqueous polymerization.These co-polymers were characterized with FTIR technique to confirm the co-polymer formation.Fluorescence recovery after photobleaching(FRAP) technique was used to investigate the diffusion behavior of fluorescein isothiocyanate labelled bovine serum albumin(FITC-BSA) in these co-polymer hydrogels,and a confocal laser scanning microscopy was applied to obtain high resolution images in real time.The results showed that the diffusion coefficient of FITC-BSA was influenced by the ratio between the acrylamide and acrylic acid in co-polymer and the co-polymer hydrogels synthesized may have potential application in controlled protein delivery system.

Key words: poly(acrylamide-co-acrylic acid), fluorescence recovery after photobleaching(FRAP) technique, fluorescein isothiocyanate labelled bovine serum albumin(FITC-BSA), diffusion coefficient

CLC Number:

CUI Jin-lei, ZHOU Wen-wen, QIU Yu-qin, GAO Yun-hua. Study on the Protein Diffusion Behavior in the Poly(Acrylamide-co-Acrylic Acid) Copolymer by FRAP Technique[J]. Imaging Science and Photochemistry, 2011, 29(5): 336-343.

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Study on the Protein Diffusion Behavior in the Poly(Acrylamide-co-Acrylic Acid) Copolymer by FRAP Technique

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