Synthesis of Pure β-Ca3(PO4)2 Bioceramics with Gelatin by Precipitation Method

doi:10.7517/j.issn.1674-0475.2012.04.269

Abstract

Abstract: Pure β-tricalcium phosphate (β-TCP) was synthesized using the precipitation method, starting from the gelatin, Ca (NO₃)₂ and (NH₄)₂HPO₄ precursors by adopting the initial Ca/P ratio of 1.5. FT-IR spectroscopy and X-ray diffractometry indicated the solution derived precipitate was calcium deficient phosphate (Ca-dHAP) and it would thermally transform into pure β-TCP as the amount of gelatin exceeds 0.22% (mass concentration). The Ca-dHAP crystal dimensions decreased with increasing gelatin amount as shown by crystallite calculation and SSA measurement. TEM results showed that the as prepared Ca-dHAP powders were needle-like and formed a botryoidal shape characteristic of β-TCP when being calcined at high temperatures. The DTA/TG results showed that gelatin chemically bonded with the Ca-dHAP crystals which could be beneficial for the absorption of water molecules and the absorbed water molecules, then subsequently reacted with Ca-dHAP to form HA which would react with a second phase calcium pyrophosphate (Ca₂P₂O₇) (CPP) to prepare pure β-TCP. In this paper, we also studied the mechanism of gelatin on the formation of β-TCP.

Key words: gelatin, pure β-TCP, Ca-dHAP, bone repair, precipitation

CLC Number:

MA Ming, WANG Xue-song, ZHANG Bing, GUO Yan-chuan. Synthesis of Pure β-Ca₃(PO₄)₂ Bioceramics with Gelatin by Precipitation Method[J]. Imaging Science and Photochemistry, 2012, 30(4): 269-279.

References

[1] Dorozhkin SV, Epple M. Biological and medical significance of calcium phosphates[J]. Angewandte Chemie-International Edition, 2002, 41(17): 3130-3146.
[2] Yuan H, Groot K. In: Reis R L, Weiner S, editors. Calcium Phosphate Biomaterials: An Overview[M]. Netherlands: Springer, 2005. 37-57.
[3] Hench L L. Bioceramics - from Concept to Clinic[J]. Journal of the American Ceramic Society, 1991, 74(7): 1487-1510.
[4] Stankewich C J, Swiontkowski M F, Tencer A F, et al. Augmentation of femoral neck fracture fixation with an injectable calcium-phosphate bone mineral cement[J]. Journal of Orthopaedic Research, 1996,14(5): 786-793.
[5] Ducheyne P, Cuckler J M. Bioactive ceramic prosthetic coatings[J]. Clinical Orthopaedics and Related Research, 1992, 276:102-114.
[6] Grundel R E, Chapman M W, Yee T, Moore D C. Autogenic bone-marrow and porous biphasic calcium phosphate ceramic for segmental bone defects in the canine ulna[J]. Clinical Orthopaedics and Related Research, 1991, 266: 244-258.
[7] Lu J, Descamps M, Dejou J, et al. The biodegradation mechanism of calcium phosphate biomaterials in bone[J]. Journal of Biomedical Materials Research, 2002, 63(4): 408-412.
[8] Gauthier O, M黮ler R, von Stechow D, et al. In vivo bone regeneration with injectable calcium phosphate biomaterial: A three-dimensional micro-computed tomographic, biomechanical and SEM study[J]. Biomaterials, 2005,26(27): 5444-5453.
[9] Kivrak N, Tas A C. Synthesis of calcium hydroxyapatite-tricalcium phosphate (HA-TCP) composite bioceramic powders and their sintering behavior[J]. Journal of the American Ceramic Society, 1998,81(9): 2245-2252.
[10] Murugan R, Ramakrishna S. Crystallographic study of hydroxyapatite bioceramics derived from various sources[J]. Crystal Growth & Design, 2004, 5(1): 111-112.
[11] Hench L L. Bioceramics[J]. Journal of the American Ceramic Society, 1998, 81(7): 1705-1728.
[12] Rejda B V, Peelen J G J, Groot K D. Tricalcium phosphate as a bone substitution[J]. Journal of Bioengineering, 1977, 1(2): 93-97.
[13] Braye F, Irigaray J L, Jallot E, et al. Resorption kinetics of osseous substitute: Natural coral and synthetic hydroxyapatite[J]. Biomaterials, 1996,17(13): 1345-1350.
[14] Bow J S, Liou S C, Chen S Y. Structural characterization of room-temperature synthesized nano-sized [beta]-tricalcium phosphate[J]. Biomaterials, 2004, 25(16): 3155-3061.
[15] Okazaki M, Sato M. Computer graphics of hydroxyapatite and [beta]-tricalcium phosphate[J]. Biomaterials, 1990, 11(8): 573-578.
[16] Pang Y X, Bao X. Influence of temperature, ripening time and calcination on the morphology and crystallinity of hydroxyapatite nanoparticles[J]. Journal of the European Ceramic Society, 2003, 23(10): 1697-1704.
[17] Mostafa N Y. Characterization, thermal stability and sintering of hydroxyapatite powders prepared by different routes[J]. Materials Chemistry and Physics, 2005, 94(2-3): 333-341.
[18] Liou S C, Chen S Y. Transformation mechanism of different chemically precipitated apatitic precursors into [beta]-tricalcium phosphate upon calcination[J]. Biomaterials, 2002, 23(23): 4541-4547.
[19] Cuneyt Tas A, Korkusuz F, Timucin M, et al. An investigation of the chemical synthesis and high-temperature sintering behaviour of calcium hydroxyapatite (HA) and tricalcium phosphate (TCP) bioceramics[J]. Journal of Materials Science: Materials in Medicine, 1997,8(2): 91-96.
[20] Ishikawa K, Ducheyne P, Radin S. Determination of the Ca/P ratio in calcium-deficient hydroxyapatite using X-ray diffraction analysis[J]. Journal of Materials Science: Materials in Medicine, 1993,4(2): 165-168.
[21] Raynaud S, Champion E, Bernache A D, et al. Calcium phosphate apatites with variable Ca/P atomic ratio I.Synthesis, characterisation and thermal stability of powders[J]. Biomaterials, 2002,23(4): 1065-1072.
[22] Raynaud S, Champion E, Bernache A D. Calcium phosphate apatites with variable Ca/P atomic ratio II. Calcination and sintering[J]. Biomaterials, 2002, 23(4): 1073-1080.
[23] Kikuchi M, Itoh S, Ichinose S, et al. Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo[J]. Biomaterials, 2001,22(13): 1705-1711.
[24] Hartgerink J D, Beniash E, Stupp S I. Self-assembly and mineralization of peptide-amphiphile nanofibers[J]. Science, 2001,294(5547): 1684-1688.
[25] Mortier A, Lemaitre J, Rouxhet P G. Temperature-programmed characterization of synthetic calcium-deficient phosphate apatites[J]. Thermochimica Acta, 1989,143: 265-282.

Synthesis of Pure β-Ca₃(PO₄)₂ Bioceramics with Gelatin by Precipitation Method

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