A Turn-on Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine B

doi:10.7517/j.issn.1674-0475.2015.03.195

Abstract

Abstract:

As a dicarboxyl metabolite produced by living cells, α-ketoglutaratc acid (α-KA) can be converted to 2-hydroxyglutarate (2HG) which is confirmed to be associated with the acute myeloid leukemia (AML). In this paper, a rhodamine B-based turn-on fluroescent sensor for α-KA has been designed and synthesized, which is consist of rhodamine-B and hydrazino group. After a series of parallel optimization such as response time, pH and temperature, RBN shows an obvious turn-on fluorescence response to α-KA. In addition, the sensor exhibits good selectivity towards α-KA among 20 common amino acids, various dicarbonyl derivatives and reactive oxygen species.

Key words: rhodamine B, α-ketoglutaric acid, fluroescence probe, turn-on

JIAO Changhong, HE Ye, JIN Pengwei, ZHU Shiqin, ZHU Weihong. A Turn-on Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine B[J]. Imaging Science and Photochemistry, 2015, 33(3): 195-202.

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