Imaging Science and Photochemistry ›› 2016, Vol. 34 ›› Issue (1): 23-29.DOI: 10.7517/j.issn.1674-0475.2016.01.023

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Development and Application of Digital Holographic Microscopy in Biomedicine

WU Yumin1,2, SONG Guoqing1,2, FENG Yunpeng1,2, WANG Zhong3   

  1. 1. Joint Research Center for Optomechatronics Engineering, School of Optoelectronics, Beijing Institute of Technology, Beijing 100081, P. R. China;
    2. Shenzhen Research Institute, Beijing Institute of Technology, Shenzhen 518057, Guangdong, P. R. China;
    3. Boruihuida Science and Technology Ltd. Co., Shenzhen 518057, Guangdong, P. R. China
  • Received:2015-10-23 Revised:2015-11-04 Online:2016-01-15 Published:2016-01-15


In recent years, with the rapid development of optoelectronic imaging devices such as CCD and computer technology, digital holographic microscopy technology has been developed, which has been widely used in the field of biomedicine with the characteristics of no-contact, quantitative, three-dimensional imaging.The development of digital holographic microscopy is reviewed.The advantages and disadvantages of digital holographic microscopy and other techniques are compared.From the point of view of light source, the important influence of different light source on digital holographic microscopy imaging and the corresponding algorithm are discussed.The latest applications of digital holographic microscopy in biomedicine are enumerated.

Key words: digital holography, image processing, biomedicine, microscopic imaging