胶原/明胶的结构(构象)与性能研究——Ⅱ.酸溶胶原(SLK)的四种不同构象的分离

doi:10.7517/j.issn.1674-0475.1993.03.204

影像科学与光化学 ›› 1993, Vol. 11 ›› Issue (3): 204-208.DOI: 10.7517/j.issn.1674-0475.1993.03.204

胶原/明胶的结构(构象)与性能研究——Ⅱ.酸溶胶原(SLK)的四种不同构象的分离

金桂仙, 彭必先

中国科学院感光化学研究所北京 100101

收稿日期:1992-04-30 修回日期:1993-02-28 出版日期:1993-08-20 发布日期:1993-08-20
通讯作者: 彭必先

STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLL AGE/GELATINⅡ. THE SEPARATION OF DIFFERENT CONFORMATIONS IN ACLD——SOLUBLE COLLAGEN

JIN GUI-XJAN, PENG BI-XIAN

Institute of Photographic Chemistry, Academia Sinica, Beijing 100101, P. R. China

Received:1992-04-30 Revised:1993-02-28 Online:1993-08-20 Published:1993-08-20

摘要/Abstract

摘要： 本文比较详细地描述了CM-52离子交换层析的实验方法。用这一离子交换层析的方法将Ⅰ型酸溶胶原中四种不同构象α₁、β₁₁、β₁₂和α₂,成功地分离为四个不同的级份,其中α₁还分成为α_1a和α_1b。详细讨论了影响分离效果的各种因素,确定了最佳的分离条件。

关键词: 酸溶胶原, 构象分离, 离子交换层析

Abstract: The expermental method of CM-52 ionic chromatography was described in details. Using this method, 4 different conformations contained in acid-soluble collagen have been successfully eluted into 4 separated bands. The various factors affecting the separation were discussed and the optimum separation conditions used in present study have been selected and established.

Key words: acid-soluble collagen, conformation separation, ionic exchange cbro-matograghy

金桂仙, 彭必先. 胶原/明胶的结构(构象)与性能研究——Ⅱ.酸溶胶原(SLK)的四种不同构象的分离[J]. 影像科学与光化学, 1993, 11(3): 204-208.

JIN GUI-XJAN, PENG BI-XIAN. STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLL AGE/GELATINⅡ. THE SEPARATION OF DIFFERENT CONFORMATIONS IN ACLD——SOLUBLE COLLAGEN[J]. Imaging Science and Photochemistry, 1993, 11(3): 204-208.

[1]	傅晓敏, 陈丽娟, 彭必先. 胶原中不同构象组份的水解性能研究[J]. 影像科学与光化学, 1997, 15(1): 25-31.
[2]	金桂仙, 彭必先. 胶原/明胶的结构(构象)与性能研究Ⅲ.从酸溶胶原(SLK)中分离出的四种不同构象的表征[J]. 影像科学与光化学, 1993, 11(4): 302-306.

胶原/明胶的结构(构象)与性能研究——Ⅱ.酸溶胶原(SLK)的四种不同构象的分离

STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLL AGE/GELATINⅡ. THE SEPARATION OF DIFFERENT CONFORMATIONS IN ACLD——SOLUBLE COLLAGEN

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