影像科学与光化学 ›› 1993, Vol. 11 ›› Issue (4): 302-306.DOI: 10.7517/j.issn.1674-0475.1993.04.302

• 研究论文 • 上一篇    下一篇

胶原/明胶的结构(构象)与性能研究Ⅲ.从酸溶胶原(SLK)中分离出的四种不同构象的表征

金桂仙, 彭必先   

  1. 中国科学院感光化学研究所, 北京 100101
  • 收稿日期:1992-04-30 修回日期:1993-02-28 出版日期:1993-11-20 发布日期:1993-11-20
  • 通讯作者: 彭必先

STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLLAGEN/GELATIN Ⅲ.THE CHARACTERIZATION OF 4 DIFFERENT CONFORMATIONS SEPARATED FROM COLLAGEN

JIN GUI-XIAN, PENG BI-XIAN   

  1. Institute of Photographic Chemistry, Acadcmia Sinica, Beijing 100101, P. R. China
  • Received:1992-04-30 Revised:1993-02-28 Online:1993-11-20 Published:1993-11-20

摘要: 本文以高纯α1α2β11β12和γ为标准样品,测定了它们在SDS-PAGE电泳中的迁移距离,利用这些组份的分子量的对数log Mn与迁移距离的线性关系,对从酸溶胶原中分离出来的a、b,c、d、e和f六个不同级份进行了鉴定,表明从a到f的六个不同的分离级份是α<sub>1a,α1b,β11β12,αz,和(α1+β11)。这一分离是成功的。其中α1αα1b是首次分离成功。

关键词: 胶原, 明胶, CM-52离子交换层析, 构象分离

Abstract: Chemical reagent of high purity α1,α2,β11,β12 and γ were collected and used as the standards.Their migration distances (d) in SDS-PAGE have been determined.Using the linear relationship between the logarithum of molecular weight log Mn and d, the 6 different fractions (a, b, c, d, e and f)have been separated from collagen, desalted, lyophilied and identified as α1a,α1b,β11,β12,α2 and (α1 +β11) respectively.

Key words: collagen, conformation, separation, SDS-PAGE