长碳链正电糖甙和葡萄糖氧化酶混合Langmuir-Blodgett膜的光谱研究

doi:10.7517/j.issn.1674-0475.1998.01.32

影像科学与光化学 ›› 1998, Vol. 16 ›› Issue (1): 32-37.DOI: 10.7517/j.issn.1674-0475.1998.01.32

长碳链正电糖甙和葡萄糖氧化酶混合Langmuir-Blodgett膜的光谱研究

杜玉扣¹, 唐季安¹, 江龙¹, Paul Boulanger ²

1. 中国科学院感光化学研究所, 北京 100101;
2. 里昂大学法国科学研究院有机化学实验室, 法国

收稿日期:1997-04-14 修回日期:1997-07-16 出版日期:1998-02-20 发布日期:1998-02-20
通讯作者: 江龙
基金资助:
中国科学院重大基础研究和国家自然科学基金资助项目(批准号:29573139)

A SPECTROSCOPY STUDY OF THE MIXED LANGMUIR BLODGETT FILMS OF ALKYL GLYCOSIDE WITHPOSITIVE CHARGE AND GLUCOSE OXIDASE

DU Yukou¹, TANG Ji’an¹, JIANG Long¹, Paul Boullanger²

1. Institute of Photographic Chemistry, The Chinese Academy of Sciences, Beijing 100101, P. R. China;
2. Laboratoire Chimie Organique, URA CNRS 463, Universite Lyon 1, France

Received:1997-04-14 Revised:1997-07-16 Online:1998-02-20 Published:1998-02-20

摘要/Abstract

摘要： 合成了简称C₁₈的十八烷基-2-氨基-2-脱氧-β-D-吡喃葡萄糖甙。通过π-A曲线研究了C₁₈和GOD(葡萄糖氧化酶)的相互作用,并用光谱方法研究了转移的C₁₈/GOD混合LB膜的光谱特性。从圆二色性(CD)光谱结果可以看出,固定在C₁₈膜里的GOD二级结构发生了变化。低温荧光实验显示混合膜中一部分GOD仍然保持其天然结构,但另一些则发生了部分变性。

关键词: 葡萄糖氧化酶, 圆二色谱, 荧光光谱, LB膜

Abstract: Alkyl 2-amino-2-deoxy-β-D-glucopyranoside called C₁₈ was synthesized. The interaction between C₁₈and GOD (glucose oxidase) was studied by recording isotherms of monolayers. The transferred mixed C₁₈/GOD LB films were investigated by means of spectroscopy methods. From the results of CD (circular dichroism) spectrum,GOD immobilized in C₁₈films will partly change their secondary structure. According to the low temperature fluorescence experiment,part of GOD in the LB film keeps native structure,others of them become partly denatured.

Key words: glucose oxidase, circular dichroism spectrum, fluorescence spectrum, LangmuirBlodgett(LB) film

杜玉扣, 唐季安, 江龙, Paul Boulanger . 长碳链正电糖甙和葡萄糖氧化酶混合Langmuir-Blodgett膜的光谱研究[J]. 影像科学与光化学, 1998, 16(1): 32-37.

DU Yukou, TANG Ji’an, JIANG Long, Paul Boullanger. A SPECTROSCOPY STUDY OF THE MIXED LANGMUIR BLODGETT FILMS OF ALKYL GLYCOSIDE WITHPOSITIVE CHARGE AND GLUCOSE OXIDASE[J]. Imaging Science and Photochemistry, 1998, 16(1): 32-37.

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长碳链正电糖甙和葡萄糖氧化酶混合Langmuir-Blodgett膜的光谱研究

A SPECTROSCOPY STUDY OF THE MIXED LANGMUIR BLODGETT FILMS OF ALKYL GLYCOSIDE WITHPOSITIVE CHARGE AND GLUCOSE OXIDASE

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