包覆NIPAM-ODA双亲共聚物的脂质体的温控释放行为

doi:10.7517/j.issn.1674-0475.1999.03.208

影像科学与光化学 ›› 1999, Vol. 17 ›› Issue (3): 208-216.DOI: 10.7517/j.issn.1674-0475.1999.03.208

包覆NIPAM-ODA双亲共聚物的脂质体的温控释放行为

史向阳¹, 孙曹民², 吴世康¹

1. 中国科学院感光化学研究所, 北京100101;
2. 北京理工大学生物材料实验室, 北京100081

收稿日期:1998-06-17 修回日期:1998-08-25 出版日期:1999-08-20 发布日期:1999-08-20
通讯作者: 吴世康
基金资助:
国家自然科学基金重点课题资助(课题号:29733100)

TEMPERATURE-CONTROLLED RELEASE OF AN AQUEOUS MARKER FROM LIPOSOMES COATED WITHNIPAM-ODA COPOLYMERS

SHI Xiangyang¹, SUN Caomin², WU Shikang¹

1. Institute of Photographic Chemistry, The Chinese Academy of Sciences, Beijing 100101, P.R. China;
2. Laboratory of Biomaterials, Beijing Institute of Technology, Beijing 100081, P.R. China

Received:1998-06-17 Revised:1998-08-25 Online:1999-08-20 Published:1999-08-20

摘要/Abstract

摘要： 为了研究对温度敏感的双亲性共聚物包覆的脂质体的温控释放行为,合成了N-异丙基丙烯酰胺(NIPAM)和丙烯酸十八酯(ODA)的共聚物。利用荧光探针法研究了共聚物水溶液随温度升高时出现的LCST(lower critical solution temperature)现象,表明该高分子在温度升高到30℃以上时存在着明显的相分离行为。5(6)-羧基荧光素(5(6)-CF)为标记物,研究了高分子包覆的小单层脂质体(small unilamelar vesicles)的释放行为。发现在温度低于30℃时,5(6)-CF的释放百分率比未包覆高分子的脂质体要低;而当温度升高到30℃以上时,其释放百分率明显提高。这种温控释放行为和包覆在脂质体上的高分子在其LCST时存在的相分离行为有关。进一步利用荧光偏振法研究了脂质体膜在包覆高分子后的流动性变化,发现:在温度低于30℃时,其流动性随温度升高而增大;而在温度高于30℃时,脂质体膜流动性随温度升高而降低,进一步证实了高分子在其LCST以上时对脂质体膜的破坏作用。

关键词: 水溶性高分子, 脂质体, 温控释放, 荧光偏振

Abstract: To study the temperature-controlled release behavior of the liposome coated with an amphiphilic copolymer which is temperaturesensitive, the copolymer of N-isopropylacrylamide (NIPAM) and octadecyl acrylate (ODA) was synthesized. The phenomenon of LCST(Lower Critical Solution Temperature)of the copolymer in aqueous solution with the increase of the temperature was studied by using fluorescence probe method. Results showed that the obvious phase separation occurs when temperature was increased to 30. The release property of small unilamellar vesicles (SUV) coated with the copolymer was studied by using 5(6)-carboxyfluorescein (5(6)-CF) as a water soluble marker. Results showed that the coating of the copolymer on the liposomal surface resulted in reduction of the release below 30 and enhancement of the release above 30. It indicated that the temperature-controlled release behavior of the liposome was directly related to the phase separation behavior of the coating copolymer when temperature was increased to its LSCT. Moreover, fluorescence polarization method was used to study the influence of the coating copolymer on the fluidity of the liposomal membrane. Results showed that at temperature below 30 the fluidity of the liposomal membrane increased with the increase of temperature; however, at temperature above 30 the fluidity of the liposomal membrane decreased with the increase of temperature. It further indicated that the copolymer which was bound at the surface of the liposome can destroy the liposomal membrane when temperature was increased at or above its LCST.

Key words: water-soluble polymer, liposomes, temperature-controlled release, fluorescence polarization

史向阳, 孙曹民, 吴世康. 包覆NIPAM-ODA双亲共聚物的脂质体的温控释放行为[J]. 影像科学与光化学, 1999, 17(3): 208-216.

SHI Xiangyang, SUN Caomin, WU Shikang. TEMPERATURE-CONTROLLED RELEASE OF AN AQUEOUS MARKER FROM LIPOSOMES COATED WITHNIPAM-ODA COPOLYMERS[J]. Imaging Science and Photochemistry, 1999, 17(3): 208-216.

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包覆NIPAM-ODA双亲共聚物的脂质体的温控释放行为

TEMPERATURE-CONTROLLED RELEASE OF AN AQUEOUS MARKER FROM LIPOSOMES COATED WITHNIPAM-ODA COPOLYMERS

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