细菌视紫红质-聚乙烯醇薄膜M态衰减动力学研究

doi:10.7517/j.issn.1674-0475.2001.02.96

影像科学与光化学 ›› 2001, Vol. 19 ›› Issue (2): 96-101.DOI: 10.7517/j.issn.1674-0475.2001.02.96

细菌视紫红质-聚乙烯醇薄膜M态衰减动力学研究

李美玲, 李宝芳, 江龙

中国科学院化学所分子科学中心, 胶体与界面实验室, 北京100080

收稿日期:2000-07-19 修回日期:2000-09-19 出版日期:2001-05-20 发布日期:2001-05-20
通讯作者: 李宝芳
基金资助:
国家自然科学基金资助项目(69731010);中国科学院资助项目(KJ951-A₁-501-05)

DECAY KINETICS OF M-STATE IN BACTERIORHODOPSIN- POLY(VINYL ALCOHOL) FILMS

LI Mei-ling, LI Bao-fang, JIANG Long

Lab. Colloid and Interface, Center of Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, P.R. China

Received:2000-07-19 Revised:2000-09-19 Online:2001-05-20 Published:2001-05-20

摘要/Abstract

摘要： 利用闪光光解法和紫外可见光谱法研究了室温条件下,pH值的增加和干燥时间的延长对细菌视紫红质 (bR)-聚乙烯醇 (PVA)薄膜光循环过程中M态衰减动力学的影响。闪光光解动力学研究表明,M态的衰减随着 pH值的提高和干燥时间的延长而明显减慢,在pH =1 0和干燥时间为 72h的最佳制备条件下薄膜M态的衰减最为缓慢.紫外可见光谱法表明,M态的衰减可持续 15min以上,比自然条件下M态的寿命提高约 4个数量级.本文还探讨了最佳制备条件下细菌视紫红质-聚乙烯醇薄膜长寿命M态形成的机理.

关键词: 细菌视紫红质, 光循环, M态, 光致变色

Abstract: The influence of increase of pH and dehydration time on the decay kinetics of M-state in bacteriorhodopsin (bR)-poly(vinyl alcohol) (PVA) films was studied by UV-vis spectrophotometry and flash photolysis under room conditions. Flash photolysis study showed that the M-state lifetime was prolonged rather obviously by increasing of pH and dehydration time. The slowest decay of M-state was observed in bR-PVA film prepared at pH 10 and dehydration time of 72 h and UV-vis spectrophotometry indicated that the M-state could sustain over 15 min, which represented a four order of magnitude improvement over that of native bR. In addition, the mechanism for the prolongation of M-state in bR-PVA films was discussed in this paper.

Key words: bacteriorhodopsin, photocycle, M-state, photochromism

中图分类号:

O644

李美玲, 李宝芳, 江龙. 细菌视紫红质-聚乙烯醇薄膜M态衰减动力学研究[J]. 影像科学与光化学, 2001, 19(2): 96-101.

LI Mei-ling, LI Bao-fang, JIANG Long. DECAY KINETICS OF M-STATE IN BACTERIORHODOPSIN- POLY(VINYL ALCOHOL) FILMS[J]. Imaging Science and Photochemistry, 2001, 19(2): 96-101.

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细菌视紫红质-聚乙烯醇薄膜M态衰减动力学研究

DECAY KINETICS OF M-STATE IN BACTERIORHODOPSIN- POLY(VINYL ALCOHOL) FILMS

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