2-甲基-1,4-萘醌对DNA光敏损伤的诱导作用

doi:10.7517/j.issn.1674-0475.2002.05.352

影像科学与光化学 ›› 2002, Vol. 20 ›› Issue (5): 352-357.DOI: 10.7517/j.issn.1674-0475.2002.05.352

2-甲基-1,4-萘醌对DNA光敏损伤的诱导作用

张连文¹, 林维真², 姚思德², 林念芸²

1. 聊城师范学院, 化学系, 山东聊城, 252059;
2. 中国科学院, 上海原子核研究所辐射化学开放实验室, 上海, 800-204 邮政信箱201800

收稿日期:2001-11-23 修回日期:2002-02-14 出版日期:2002-09-23 发布日期:2002-09-23
通讯作者: 姚思德、林维真
基金资助:
中国自然科学基金委"九五"重点项目(39830090);中国科学院"九五"重点项目(KJ952-S1-419)

DNA PHOTOSENSITIZATION DAMAGE INDUCED BY 2-METHY-1,4-NAPHTHONINONE

ZHANG Lian-wen¹, LIN Wei-zhen², YAO Si-de², LIN Nian-yun²

1. Department of Chemistry, Liaocheng Teachers University, Shandong Liaocheng 252059, P. R. China;
2. Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, P. R. China

Received:2001-11-23 Revised:2002-02-14 Online:2002-09-23 Published:2002-09-23

摘要/Abstract

摘要： 选择波长337 nm的激光作为激励光源,借助凝胶电泳研究了2-甲基-1,4-萘醌诱导的DNA光敏损伤.结果表明:在氧气饱和、脱氧条件下光敏损伤显著,DNA损伤主要与光子剂量、核酸与萘醌浓度比及DNA存在形式有关.

关键词: 2-甲基-1,4-萘醌, 光敏损伤, 琼脂糖凝胶电泳

Abstract: By laser irradiation(λ=337 nm) and argarose gel electrophoresis,DNA photosensitization damage induced by 2-methyl-1,4-naphthoninone(MQ) was studied in this paper.Both samples saturated with N₂ and O₂ can be damaged markedly,this damage was prominently related to the concentration ratio of DNA/MQ,the photon dosage and DNA form.

Key words: 2-methyl-1,4-naphthoninone(MQ), photosensitization damage, argarose gel electrophoresis

中图分类号:

张连文, 林维真, 姚思德, 林念芸. 2-甲基-1,4-萘醌对DNA光敏损伤的诱导作用[J]. 影像科学与光化学, 2002, 20(5): 352-357.

ZHANG Lian-wen, LIN Wei-zhen, YAO Si-de, LIN Nian-yun. DNA PHOTOSENSITIZATION DAMAGE INDUCED BY 2-METHY-1,4-NAPHTHONINONE[J]. Imaging Science and Photochemistry, 2002, 20(5): 352-357.

参考文献

[1] Loeff I,Kan Y,Armitage B,et al.Interactions of formate ion with triplet of anthraquinone,1,4-naphthoquinone,benzophenone-4-carboxylate and benzophenone-4-sulfonate[J].J.Phys.Chem.,1991,95:4423-4430.
[2] Fisher G,Land E J.Photosensitization of pyrimidines by 2-methylnathoquinone in water:a laser flash photolysis study[J].Photochem.Photobiol.,1983,37:27-32.
[3] Patel K B,Willson R L.Semiquinone free radicals and oxygen.Pulse radiolysis study of one electron transfer equilibria[J].J.Chem.Soc.,Faraday Trans.,1973,69:814-825.
[4] Wagner J R,Vanlier J E,Johnston L J.Quinone sensitized electron transfer photooxidation of nucleic acids:chemistry of thymine and thymidine radical cations in aqueous solution[J].Photochem.Photobiol.,1990,52:333-343.
[5] Fisher G J,Watts M E,Patel K B,et al.Sensitization of ultraviolet radiation damage in bacterial and mammalian cells[J].Br.J.Cancer,1978,37:Suppl.Ⅲ.111-114.
[6] Wagner J R,Cadet J E,Fisher G J.Photooxidation of thymine sensitized by 2-methyl-1,4-naphthoquinone:analysis of products including three novel photodimers[J].Photochem.Photobiol.,1984,40:589-597.
[7] Daniele Praseuth,Alain Gaudemer,Jean-Baptiste Verlhac,et al.Photocleavage of DNA in the presence of synthetic water-soluble porphyrins[J].Photochem.Photobiol.,1986,6:717-724.
[8] Changyuan Lu,Weizhen Lin,Wenfeng Wang,et al.Riboflavin(Vb2) photosensitized oxidation of 2’-deoxyguanosine-5’-momphosphate(dGMP) in aqueous solution:a transient intermediates study[J].Phys.Chem.Chem.Phys.,2000(2):329-335.
[9] Lee P C C,Rodgers M A J.Laser flash photokinetic studies of rose bengal sensitized photodynamic interactions of nucleotides and DNA[J].Photochem.Photobiol.,1987,45:79-86.
[10] Chim Shen,Christopher S Foote.Reactivity toward singlet oxygen of a 7,8-dihydro-8-oxoguanosine (8-hydroxyguanosin) formed by photooxidation of a guanosine derivative[J].J.Am.Chem.Soc.,1995,117:6439-6442.
[11] 高远红,杨伟志,徐国镇.肿瘤乏氧问题的研究现状[J].国外医学肿瘤学分册,1999,26(5):270-273.Gao Y H,Yang W Z,Xu G Z.The study on hypoxia problem of tumor[J].Foreign Medical Sciences Section on Oncology,1999,26(5):270-273.
[12] J Martin Brown,Amato J Giaccia.The unique physiology of solid tumors:opportunities (and problems) for cancer therapy[J].Cancer Research,1998,58:1408-1416.

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2-甲基-1,4-萘醌对DNA光敏损伤的诱导作用

DNA PHOTOSENSITIZATION DAMAGE INDUCED BY 2-METHY-1,4-NAPHTHONINONE

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