Ru(phen)32+-SDBS-KMnO4化学发光体系检测半胱氨酸的研究

doi:10.7517/j.issn.1674-0475.2005.04.267

影像科学与光化学 ›› 2005, Vol. 23 ›› Issue (4): 267-273.DOI: 10.7517/j.issn.1674-0475.2005.04.267

Ru(phen)₃²⁺-SDBS-KMnO₄化学发光体系检测半胱氨酸的研究

张韶虹^1,2, 郗娟¹, 何治柯¹

1. 武汉大学化学与分子科学学院湖北武汉430072;
2. 襄樊职业技术学院湖北襄樊441021

收稿日期:2005-01-31 修回日期:2005-04-18 出版日期:2005-07-23 发布日期:2005-07-23
通讯作者: 何治柯,男,Email: zhkhe@whu.edu.cn, Tel: 027-87162672.
基金资助:
国家自然科学基金项目(20275028)．

Chemiluminescence Determination of Cysteine with Ru(phen)₃²⁺ -SDBS-KMnO₄ System

ZHANG Shao-hong^1,2, XI Juan¹, HE Zhi-ke¹

1. College of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, Hubei, P. R. China;
2. Xiangfan Vocational and Technical College, Xiangfan 441021, Hubei, P. R. China

Received:2005-01-31 Revised:2005-04-18 Online:2005-07-23 Published:2005-07-23

摘要/Abstract

摘要： 在20mmol·L^-1硫酸-1.2mmol·L^-1十二烷基苯磺酸钠(SDBS)介质中,半胱氨酸可以增强三(1,10-菲咯啉)钌(Ⅱ)[Ru(phen)₃²⁺]-KMnO₄化学发光体系的发光强度.基于此,建立了一种化学发光直接检测半胱氨酸的新方法.在优化的实验条件下,该方法的线性范围和检测限分别为2.5×10^-2-2.0μg·mL^-1和2.1×10^-2μg·mL^-1,对11份含半胱氨酸0.5μg·mL^-1的溶液平行测定的相对标准偏差(R.S.D.)为5.3%.将其用于合成样品中半胱氨酸含量的测定,结果令人满意.并提出了可能的化学发光机理.

关键词: 半胱氨酸, 化学发光, 三(1,10-菲咯啉)钌(Ⅱ)

Abstract: In the presence of anionic surfactant, sodium dodecyl benzene sulfonate (SDBS), the chemiluminescence (CL) intensity of tris-(1,10-phenanthroline)ruthenium(Ⅱ)( Ru(phen)₃²⁺)-KMnO₄ system could be greatly enhanced by Cysteine, based on which, a novel CL method for the determination of Cysteine was developed. Under the optimum experimental conditions, the linear range and detection limit of the proposed method were 2. 5×10^-2-2.0μg·mL^-1 and 2.1×10^-2 μg·mL^-1, respectively. The relative standard deviation (R. S. D.) of determining 11 solutions containing 0.5 μg·mL^-1 Cysteine was 5.3%. This CL method has been successfully applied to the determination of Cysteine in the synthetic samples. The mechanism of CL reaction was also studied.

Key words: cysteine, chemiluminescence, tris-(1,10-phenanthroline)ruthenium(Ⅱ)

中图分类号:

O657

张韶虹, 郗娟, 何治柯. Ru(phen)₃²⁺-SDBS-KMnO₄化学发光体系检测半胱氨酸的研究[J]. 影像科学与光化学, 2005, 23(4): 267-273.

ZHANG Shao-hong, XI Juan, HE Zhi-ke. Chemiluminescence Determination of Cysteine with Ru(phen)₃²⁺ -SDBS-KMnO₄ System[J]. Imaging Science and Photochemistry, 2005, 23(4): 267-273.

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Ru(phen)₃²⁺-SDBS-KMnO₄化学发光体系检测半胱氨酸的研究

Chemiluminescence Determination of Cysteine with Ru(phen)₃²⁺ -SDBS-KMnO₄ System

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