绿色荧光蛋白质(GFP)的发现、表达和发展

doi:10.7517/j.issn.1674-0475.2009.01.69

影像科学与光化学 ›› 2009, Vol. 27 ›› Issue (1): 69-78.DOI: 10.7517/j.issn.1674-0475.2009.01.69

• 综述 • 上一篇

绿色荧光蛋白质(GFP)的发现、表达和发展

吴世康

中国科学院理化技术研究所

收稿日期:2008-11-01 修回日期:2008-11-24 出版日期:2009-01-23 发布日期:2009-01-23

The Discovery and Development of the Green Fluorescent Protein

WU Shi-kang

Technical Institute of Physics and Chemistry, Chinese A cademy of Scieces, Beijing 100190, P.R.China

Received:2008-11-01 Revised:2008-11-24 Online:2009-01-23 Published:2009-01-23

摘要/Abstract

摘要： 本文简要地介绍了2008年Nobel化学奖——绿色荧光蛋白质的主要学术内容,包括绿色荧光蛋白质的发现、发展和工作的重要意义.类-绿色荧光蛋白质可用于在时间和空间上监视越来越多的活细胞中的现象和机制,如基因表达、蛋白质的定位和动态学等.因此可以说,绿色荧光蛋白质的发现是联系到生物科学上的一次技术革命.

关键词: Nobel化学奖, 绿色荧光蛋白质, AequoreaVictoria水母

Abstract: In this paper,the principle subject of the Nobel’s Prize in Chemistry in 2008—The Green Fluorescent protein has been introduced briefly.It consists of the discovery,development and the deep significance of this subject.The GFP-like proteins can use to monitor in time and space of an ever-increasing number of phenomena in living cells and organisms like gene expression,protein localization and dynamics.So,it is able to say that the discovery of GFP is concerned with a technical revolution in Biological science.

Key words: Green Fluorescent protein, Nobel’s Prize in chemistry, Jellyfish Aequorea Victoria

中图分类号:

吴世康. 绿色荧光蛋白质(GFP)的发现、表达和发展[J]. 影像科学与光化学, 2009, 27(1): 69-78.

WU Shi-kang. The Discovery and Development of the Green Fluorescent Protein[J]. Imaging Science and Photochemistry, 2009, 27(1): 69-78.

参考文献

[1] Shimomura O,Johnson F H,Saiga Y.Extraction,purification and properties of aequorin,a bioluminescent protein from the luminous hydromedusan,Aequorea[J].J.Cell.Comp.Physiol,1962,59:223-239.
[2] Cody C W,Prasher D C,et al.Chemical structure of the kexapeptide chromophore of the Aequorea green fluorescent protein[J].Biochemistry,1993,32:1212-1218.
[3] Tsien R Y.The green fluorescent protein[J].Annu.Rev.Biochem,1998,67:509-544.
[4] Brejc K,Sixma TK,Kitts PA,Kain S R,Tsien RY,Orm M,Remington J.Structural basis for dual excitation and photoisomerization of the Aequorea victoria green-fluorescent-protein[J].PNAS,1997,94(6):2306-2311.
[5] Heim R,Prasher DC,Tsien RY.Wavelength mutations and posttranslational autoxidation of green fluorescent protein[J].PNAS,1994,91(26):12501-12504.
[6] Cubitt A B,Heim R,Adams S R,Boyd A E,Gross L A,Tsien R Y.Understanding,improving and using green fluorescent proteins[J].Trends Biochem.Sci.,1995,20:448.
[7] Chalfie M,Tu Y,Euskirchen G,Ward W W,Prasher D C.Green fluorescent protein as a marker for gene expression[J].Science,263(5148):802-805.
[8] Wang S X,Hazelrigg T.Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis[J].Nature,1994.369:400-403.
[9] Matz M V,Lukyanov K A,Lukyanov S A.My favorite molecule-Family of the green fluorescent protein:Journey to the end of the rainbow[J].BioEssay,2002,24:953.
[10] Matz MV,et al.Fluorescent proteins from nonbioluminescent anthozoan species' Nature[J].Biotechnology,1999,17:969.
[11] Campbell R E,Oded T,Palmer A E,Steinbach P A.,Baird G S,Zacharias D A,Tsien R Y.A monomeric red fluorescent protein[J].PNAS,2002,99:7877.
[12] Lippincott-Schwartz J,Snapp E,Kenworthy A.Studying protein dynamics in living cells[J].Nature Cell Biology Reviews,2001,2:444-456.
[13] Ando R,Hama H,Yamamoto-Hino M,Mizuno H,Miyawaki A.An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein[J].Proc.Natl.Acad.Sci.USA,2002,99:12651-12656.
[14] Davenport D,Nicol J A C.Luminescence in hydromedusae[C].Proc.R.Soc.(Ser B Biol Sci),London,1955.144,399-411.
[15] Shimomura O,Johnson F H,Saiga Y.Extraction,Purification and Properties of Aequorin,a Bioluminescent Protein from the Luminous Hydromedusan,Aequorea[J].J.Cell.Comp.Physiol.,1962,59:223.
[16] Cormier M J K,Hori Y D,Karkhanis J M,Anderson J E,Wampler J G M,Hastings J W.Evidence for similar biochemical requirements for bioluminescence among the coelenterates[J].J.Cell.Physiol.,1973,81:291-298.
[17] Prasher DC,Eckenrode V K,Ward W W,et al.Primary structure of the Aequores victoria green-fluorescent protein[J].Gene,1992,111:229-233.
[18] Chalfie M,Tu Y,Euskirchen G,Ward W W,Prasher D C.Green fluorescent protein as a marker for gene expression[J].Science,1994,263:5148,802-805.
[19] Heim R,Tsien R Y.Engineering green fluorescent protein for improved brightness,longer wavelengths and fluorescence energy transfer[J].Curr.Biol.,1996,6:178.
[20] Gross L A,Baird G S,Hoffman RC,Baldridge K K,Tsien R Y.The structure of the chromophore within DsRed,a red fluorescent protein from coral[J].Proc.Natl.Acad.Sci.,2000,97:11990.
[21] Baird G S,Zacharias D A,Tsien R Y.Biochemistry,mutagenesis,and oligomerization of DsRed,a red fluorescent protein from coral[J].Proc.Natl.Acad.Sci.,2000,97:11984.

绿色荧光蛋白质(GFP)的发现、表达和发展

The Discovery and Development of the Green Fluorescent Protein

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