两亲性酞菁锌对人体肝癌细胞的光灭活作用

doi:10.7517/j.issn.1674-0475.2010.05.362

影像科学与光化学 ›› 2010, Vol. 28 ›› Issue (5): 362-367.DOI: 10.7517/j.issn.1674-0475.2010.05.362

两亲性酞菁锌对人体肝癌细胞的光灭活作用

魏彦¹, 陈伟¹, 王玉², 李涛², 毕程¹, 夏春辉²

1. 齐齐哈尔大学, 化学与化学工程学院, 黑龙江, 齐齐哈尔, 161006;
2. 齐齐哈尔医学院, 黑龙江, 齐齐哈尔, 161042

收稿日期:2010-03-22 修回日期:2010-05-15 出版日期:2010-09-23 发布日期:2010-09-23
通讯作者: 陈伟,教授,E-mail:chenwei150080@163.com.
基金资助:
黑龙江省自然科学基金重点项目(D200822)

Photoinhibition on Liver Cancer Cell of Amphiphilic Phthalocyanine Zinc

WEI Yan¹, CHEN Wei¹, WANG Yu², LI Tao², BI Cheng¹, XIA Chun-hui²

1. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, P. R. China;
2. Qiqihar Medical College, Qiqihar 161042, Heilongjiang, P. R. China

Received:2010-03-22 Revised:2010-05-15 Online:2010-09-23 Published:2010-09-23

摘要/Abstract

摘要： 采用四甲基偶氮唑蓝比色法(MTT法),考察了两亲性α-四(对-羧基苯氧基)酞菁锌(Ⅱ)光敏剂对Bel-7402人体肝癌细胞的离体光动力学行为,研究了浓度和光照时间对光灭活作用的影响.结果发现:在适宜的浓度下,酞菁锌光敏剂表现出了良好的抑制作用,且此抑制过程遵循一级反应的动力学规律.另外,酞菁在光催化下产生的单重态氧能损坏癌变的细胞膜,导致靶对细胞的死亡.

关键词: 两亲性酞菁锌, Bel-7402肝癌细胞, 光灭活作用

Abstract: In vitro photodynamic activities of amphiphilic α-tetra-(p-carboxyphenoxy)phthalocyanine zinc(Ⅱ) complex toward human Bel-7402 liver cancer cell were investigated by MTT method.The photoinactivation was influenced by concentration and light irradiation time was studied.This zinc phthalocyanine as one of photosensitizers exhibits good inhibition effects under appropriate concentration,and the inhibition process follows first order reaction rule approximately.In addition,singlet oxygen species generated by phthalocyanine under illumination could wreck the cancer cell membrane,resulting in death of target cell.

Key words: amphiphilic phthalocyanine zinc, Bel-7402 liver cancer cell, photoinhibition

中图分类号:

O61
R730

魏彦, 陈伟, 王玉, 李涛, 毕程, 夏春辉. 两亲性酞菁锌对人体肝癌细胞的光灭活作用[J]. 影像科学与光化学, 2010, 28(5): 362-367.

WEI Yan, CHEN Wei, WANG Yu, LI Tao, BI Cheng, XIA Chun-hui. Photoinhibition on Liver Cancer Cell of Amphiphilic Phthalocyanine Zinc[J]. Imaging Science and Photochemistry, 2010, 28(5): 362-367.

参考文献

[1] Ackroyd R,Brown N J,Davis M F,et al.Aminolaevulinic acid-induced photodynamic therapy in the treatment of dysplastic barrett's oesophagus and adenocar[J].Laser in Medical Science,1999,14(4):275-278.
[2] 王洪武,邹珩,周云芝,等.光动力治疗恶性肿瘤临床研究[J].医学研究杂志,2007,36(7):35-38.Wang H W,Zou X,Zhou Y Z,et al.An clinical studys of photodynamic therapy different on malignant tumors[J].Journal of Medical Research,2007,36(7):35-38.
[3] Stefano B,Enrico C,Loredana B,et al.Zinc phthalocyanines-mediated photodynamic therapy induces cell death in adenocarcinoma cells[J].Journal of Organometallic Chemistry,2007,692:1269-1276.
[4] Kolarova H,Nevrelova P,Bajgar R,et al.In vitro photodynamic therapy on melanoma cell lines with phthal-ocyanine[J].Toxicology in Vitro,2007,21:249-253.
[5] 俞开潮,程红,金玲.光动力治疗用酞菁类光敏剂的合成研究进展[J].感光科学与光化学(影像科学与光化学),2003,21(2):138-146.Yu K C,Cheng H,Jin L.Progress of synthesizing phthalocyanines used for photodynamic therapy[J].Photographic Science and Photochemistry(Imaging Science and Photochemistry),2003,21(2):138-146.
[6] 黄剑东,刘丰冉,陈燕梅,等.四乙酰哌嗪苯氧基酞菁锌及其蛋白质复合物的制备、表征与光动力活性[J].无机化学学报,2006,22(3):435-442.Huang J D,Liu F R,Chen Y M,et al.Tetra-(acetyl piperazine phenoxy) phthalocyaninato zinc complexes and their proteins conjugates:Synthesis,characterisation and photodynamic activities[J].Chinese Journal of Inorganic Chemistry,2006,22(3):435-442.
[7] Barbara C,Gabrio R,Donata D,et al.Synthesis and antimycotic activity of new unsymmetrical substituted zinc phthalocyanines[J].Tetrahedron,2003,59:10025-10030.
[8] 牛晓宇,朱宇君,薛金萍,等.几种萘酞菁硅配合物的光动力效应的研究[J].感光科学与光化学(影像科学与光化学),2005,23(6):408-413.Niu X Y,Zhu Y J,Xue J P,et al.Study on photodynamic effect of silicon naphthalocyanine coordinated compounds[J].Photographic Science and Photochemistry(Imaging Science and Photochemistry),2005,23(6):408-413.
[9] Mosman T.Rapid colorimetric assay for cellular growth and survival:Application and cytotoxicity a ssay[J].J.Immunol Methods,1983,65:55-63.
[10] 邬洪源,陈伟,王玉,等.α-四(4-羧基苯氧基)酞菁锌的合成与抗肿瘤活性研究[J].辽宁师范大学学报(自然科学版),2009,32(1):94-97.Wu H Y,Chen W,Wang Y,et al.Study on synthesis and antineoplastic activity of α-tetra-(4-carboxy-phenoxy) phthalocyanine zinc[J].Journal of Liaoning Normal University (Natural Science Edition),2009,32(1):94-97.
[11] Ho W,Yu J C,Yu J G.Photocatalytic TiO₂/glass nanoflake array films[J].Langmuir,2005,21 (8):3486-3492.
[12] 黄紫洋,黄剑东,陈锦灿,等.含哌嗪取代酞菁金属配合物的合成及其对癌细胞的光灭活作用[J].无机化学学报,2008,24(1):55-60.Huang Z Y,Huang J D,Chen J C,et al.Metal phthalocyanines piperazine containing:Synthesis and photoinhibition on cancer cell[J].Chinese Journal of Inorganic Chemistry,2008,24(8):55-60.
[13] 许德余.肿瘤光动力疗法:原理·药物·临床导论[M].北京:中国医药科技出版社,1996.48-60.Xu D Y.Photodynamic Therapy of Tumor:Principles·Drugs·Clinical Introduction[M].Beijing:Chinese Medical Science and Technology Publishing House,1996.48-60.
[14] 徐萌.恶性肿瘤化疗及其对策[M].北京:军事医学科学出版社,2002.167.Xu M.Malignant Tumor to Chemotherapy and Countermeasures[M].Beijing:Military Medical Sciences Publishing House,2002.167.

两亲性酞菁锌对人体肝癌细胞的光灭活作用

Photoinhibition on Liver Cancer Cell of Amphiphilic Phthalocyanine Zinc

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