STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLLAGEN/GELATIN Ⅲ.THE CHARACTERIZATION OF 4 DIFFERENT CONFORMATIONS SEPARATED FROM COLLAGEN

doi:10.7517/j.issn.1674-0475.1993.04.302

Imaging Science and Photochemistry ›› 1993, Vol. 11 ›› Issue (4): 302-306.DOI: 10.7517/j.issn.1674-0475.1993.04.302

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STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLLAGEN/GELATIN Ⅲ.THE CHARACTERIZATION OF 4 DIFFERENT CONFORMATIONS SEPARATED FROM COLLAGEN

JIN GUI-XIAN, PENG BI-XIAN

Institute of Photographic Chemistry, Acadcmia Sinica, Beijing 100101, P. R. China

Received:1992-04-30 Revised:1993-02-28 Online:1993-11-20 Published:1993-11-20

Abstract

Abstract: Chemical reagent of high purity α₁,α₂,β₁₁,β₁₂ and γ were collected and used as the standards.Their migration distances (d) in SDS-PAGE have been determined.Using the linear relationship between the logarithum of molecular weight log M_n and d, the 6 different fractions (a, b, c, d, e and f)have been separated from collagen, desalted, lyophilied and identified as α_1a,α_1b,β₁₁,β₁₂,α₂ and (α₁ +β₁₁) respectively.

Key words: collagen, conformation, separation, SDS-PAGE

JIN GUI-XIAN, PENG BI-XIAN. STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLLAGEN/GELATIN Ⅲ.THE CHARACTERIZATION OF 4 DIFFERENT CONFORMATIONS SEPARATED FROM COLLAGEN[J]. Imaging Science and Photochemistry, 1993, 11(4): 302-306.

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STUDY ON THE STRUCTURE (CONFORMATION) AND PROPERTIES OF COLLAGEN/GELATIN Ⅲ.THE CHARACTERIZATION OF 4 DIFFERENT CONFORMATIONS SEPARATED FROM COLLAGEN

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