STUDY ON THE ACID EFFECT OF COPPER (Ⅱ) WITH GELATIN REACTION

doi:10.7517/j.issn.1674-0475.2000.04.323

Abstract

Abstract: The ratio of chelates of Cu²⁺ with gelatin in pH value range between 8.0 to 13.0 is determined by spectrophotometry. Result shows that the ratio increases with increasing pH value. Because the protons are removed from the gelatin molecule in this pH value range. The gelatin molecule provides many amino groups and carboxyl ions. Therefore the Cu²⁺ is a effective fluorsescence quenching agent due to a strong chelation between Cu²⁺ and gelatin molecule.

Key words: gelatin, cupric ion, ratio of chelates, fluorescence spectrum

CLC Number:

TQ57

TANG Shi-hua. STUDY ON THE ACID EFFECT OF COPPER (Ⅱ) WITH GELATIN REACTION[J]. Imaging Science and Photochemistry, 2000, 18(4): 323-328.

References

[1] 郭德威.生物无机化学概要[M].天津:天津科学技术出版社,1990.14,294～301
Guo D W. Essential of Bioinorganic Chemistry[M]. Tianjin: Tianjin Science and Technolgy Press, 1990.14,294～301
[2] 陈丽娟,彭必先.明胶大分子的照相性质研究进展,明胶科学与技术,1995,15(2):1～63;15(3):110～127
Chen L J, Peng B X. The progress in investigation on photographic properties of gelatin macromolecule [J]. The Science and Technology of Gelatin, 1995,15(2):1～63;15(3):110～127
[3] 范素月,彭必先.金属离子对溴碘化银乳剂微晶体的掺杂效应Ⅲ.Cu²⁺的掺杂[J].感光科学与光化学,1988,(3):52～56
Fan S Y, Peng B X. The doping effect of halide emulsion microcrystals Ⅲ. Cu²⁺—doping[J]. Photographic Science and Photochemistry, 1988,(3):52～56
[4] 唐世华,曾锡瑞,郑忠.铜(Ⅱ)/明胶(酶解明胶)络合物的制备及其络合形态的探讨[J].感光科学与光化学,1999,17(2):145～149
Tang S H, Zheng X R, Zheng Z. Preparation and complexing state study of copper (Ⅱ) with gelatin and its enzyme hydrolysis products[J]. Photographic Science and Photochemistry, 1999,17(2):145～149
[5] Strickland R D, Freeman M L, Gurule F T. Copper binding by proteins in alkaline solution[J]. Anal. Chem., 1961,33(4):545～552
[6] 孙崇敏,徐绪国,闫天堂.明胶荧光性能的研究[J].明胶科学与技术,1995,15(4):169～176
Sun C M, Xu X G, Yan T T. Study of the fluorescence characteristics of gelatin[J]. The Science and Technology of Gelatin, 1995,15(4):169～176
[7] 陶慰孙,等编著.蛋白质分子基础[M].北京:人民教育出版社,1982,227～231
Tao W S, et al. Basis of Protein Molecular[M]. Beijing: People's Education Press, 1982,227～231
[8] Barltrop J A, Coyle J D. 光化学原理[M].北京:清华大学出版社,1983.153,171
Barltrop J A, Coyle J D. Principles of Photochemistry[M].Beijing:Qinghua University Press, 1983.153,171
[9] Tani T, Saito M. Photographic effects of metal ions Ⅰ. Effects of Divalent metal ions upon emission and photographic processes in Silver bromide and silver iodobromide grains[J]. Photogr. Sci, Eng., 1973,17(5):434～441

[1]	WANG Yihu, CAO Xiaofeng, ZHANG Bing, WANG Jianing, ZHANG Weijie, GUO Yanchuan. The Research Progress of Methacrylamide Gelatin Hydrogel [J]. Imaging Science and Photochemistry, 2020, 38(1): 22-26.
[2]	WANG Yihu, ZHANG Bing, MA Ming, LU Weipeng, WANG Jianing, GUO Yanchuan. Synthesis of GelMA/PEGDA Hydrogel by UV Photopolymerization [J]. Imaging Science and Photochemistry, 2017, 35(4): 574-580.
[3]	DENG Qiu, LI Youqun, TANG Shihua. Interaction Between ZnS Nanoparticles and Gelatin [J]. Imaging Science and Photochemistry, 2016, 34(4): 336-345.
[4]	WANG Baiyang, XIAO Xinguang, TANG Shihua, LIU Cen. The Thermodynamics of Gelatin/Fe₂S₃ Bionanocomposites Formation and Gelatin Conformational Change [J]. Imaging Science and Photochemistry, 2014, 32(6): 505-513.
[5]	MA Ming, WANG Xue-song, ZHANG Bing, GUO Yan-chuan. Synthesis of Pure β-Ca₃(PO₄)₂ Bioceramics with Gelatin by Precipitation Method [J]. Imaging Science and Photochemistry, 2012, 30(4): 269-279.
[6]	JIANG Xiao-ming, SONG Lei, ZHAO Sui. Interaction Between Gemini Cationic Surfactants and Gelatin Investigated by Fluorescence [J]. Imaging Science and Photochemistry, 2012, 30(3): 175-181.
[7]	ZHANG Bing, SHI Jing-jing, WANG Ying, GUO Yan-chuan, CHEN Li-juan. Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process [J]. Imaging Science and Photochemistry, 2012, 30(2): 102-109.
[8]	XIAO Xin-guang, LI You-qun, TANG Shi-hua. Study on the In Situ Binding of Nano-CoS to Gelatin [J]. Imaging Science and Photochemistry, 2011, 29(4): 297-305.
[9]	WU Shi-kang, WANG Peng-fei. The Exciton Fission and the High Efficient Solar Cell [J]. Imaging Science and Photochemistry, 2011, 29(1): 1-10.
[10]	GE Jun, SUN Rui-xue, SHI Jing-jing, ZHANG Bing, CHEN Li-juan. Thermal Transition of Gelatin Microspheres with and Without Glutaraldehyde-Crosslinking [J]. Imaging Science and Photochemistry, 2009, 27(5): 334-341.
[11]	SUN Rui-xue, GUO Yan-chuan, ZHANG Bing, CHEN Li-juanCHEN Da-fu, SHI Rui, ZHOU Xin-hua. Studies on Hydroxyapatite Formation in Gelatin/Hydroxyapatite Composite Microspheres [J]. Imaging Science and Photochemistry, 2009, 27(5): 342-350.
[12]	TANG Shi-hua, HUANG Zai-yin. Biomimetic Synthesis of Acicular Nanometer HgS in the Gelatin Solution [J]. Imaging Science and Photochemistry, 2008, 26(3): 200-205.
[13]	TIAN Bao-zhu, TONG Tian-zhong, CHEN Feng, ZHANG Jin-long. Effects of Gelatin on Phase Transformation and Photocatalytic Activity of Nano-size Titanium Dioxide [J]. Imaging Science and Photochemistry, 2006, 24(2): 93-101.
[14]	TANG Shi-hua. Study of Interaction between Gelatin and Copper (Ⅱ) Using Synchronours Fluorescence Spectroscopy [J]. Imaging Science and Photochemistry, 2005, 23(2): 102-107.
[15]	LI Dong-qin, HUANG Bi-xia, YUE Jun, ZHOU Tao. Study on the Interaction Between Gelatin and Ag⁺ by XPS [J]. Imaging Science and Photochemistry, 2004, 22(3): 169-174.