Imaging Science and Photochemistry ›› 2015, Vol. 33 ›› Issue (4): 275-284.DOI: 10.7517/j.issn.1674-0475.2015.04.275

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Rapid Visual Immunoassay of Multi-biomarker for Heart Failure Based on Magnetic Particles Images

HUO Weisong1,2, ZHANG Lei1,2, GAO Yuzhe1,2, MENG Fanda1,2, GAO Yunhua1   

  1. 1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    2. University of Chinese Academy of Sciences, Beijing 100049, P. R. China
  • Received:2015-05-21 Revised:2015-05-28 Online:2015-07-16 Published:2015-07-16

Abstract:

Based on sandwich immunoassay, immuno-adsorption model and quantitative analysis of magnetic particles image on the surface of silicon chip, rapid immunoassay of multi-biomarkers for heart failure had been established.This method is simple and rapid compared to traditional ELISA.The assay time does not exceed 1 hour from antibody immobilization to final results obtained. In addition, it was easy to realize a joint measurement for NT-proBNP and cTnI on single silicon biochip.The limits of detection for NT-proBNP and cTnI were 21.8 pg/mL and 0.034 ng/mL, and the detection ranges were 21.840000 pg/mL and 0.03450 ng/mL, respectively.The assay results for NT-proBNP and cTnIwere compared using 83 serum samples from patients with different cardiovascular diseases with those on commercial analyzers and obtained a very good correlation (r>0.98). In conclusion, this rapid visual immunoassay is well suitable for the clinical detection of heart failure biomarkers.

Key words: silicon biochip, visualization, immunoassay, heart failure, biomarker