CD-DNA/GO Fluorescent Nanoprobe for Detection of miRNA-21 in situ Live Cell

doi:10.7517/j.issn.1674-0475.180104

Abstract

Abstract: By using carbon dots (CD) to label DNA as fluorophore by biological coupling method, combining with graphene oxide (GO) as quencher, CD-DNA/GO fluorescent nanoprobe was constructed, and developed for miRNA-21 detection. In CD-DNA/GO, the fluorescence of CD was quenched by GO through long-range resonance energy transfer (LrRET). In present of miRNA-21, the DNA bind with miRNA-21 to form duplex, releasing CD-DNA from GO and interrupting the LrRET, and the fluorescence of CD was recovery to achieve miRNA-21 detection by fluorescence "turn on" mode. The obtained the linear range for miRNA-21 was from 1 nmol/L to 500 nmol/L; the detection of limit was estimated to be 0.2 nmol/L by the miRNA-21 fluorescent nanoprobe. The nanoprobe was achieved for detection of miRNA-21 in situ live cell imaging, which provide the basic research for in situ diagnosis and treatment of leukemia.

Key words: carbon dots, graphene oxide, fluorescent imaging, DNA, miRNA detection

HU Xianyun, MENG Tiehong, WANG Chuanming, ZHANG Ruguo, LI Wei, LÜ Tingyong, JIANG Jiazhi, MO Xingze, LONG Youguo, YU Yuesheng, PAN Weidong. CD-DNA/GO Fluorescent Nanoprobe for Detection of miRNA-21 in situ Live Cell[J]. Imaging Science and Photochemistry, 2018, 36(4): 350-358.

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