影像科学与光化学 ›› 2002, Vol. 20 ›› Issue (5): 391-398.DOI: 10.7517/j.issn.1674-0475.2002.05.391

• 综述 • 上一篇    

嗜盐菌光敏蛋白的提纯方法

褚金芳, 唐季安   

  1. 中国科学院, 化学研究所, 分子科学中心, 胶体与界面实验室, 北京, 100080
  • 收稿日期:2001-02-25 修回日期:2002-03-26 出版日期:2002-09-23 发布日期:2002-09-23
  • 通讯作者: 唐季安,Tel:(010)64888178,E-mail:g105a@ipc.ac.cn;ji_an_tang@hotmail.com
  • 基金资助:
    973基金资助项目(0021-6)

PURIFICATION OF PHOTOSENSITIVE PROTEIN FROM HALOPHILE

CHU Jin-fang, TANG Ji-an   

  1. Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, P. R. China
  • Received:2001-02-25 Revised:2002-03-26 Online:2002-09-23 Published:2002-09-23

摘要: 对近年来文献中关于嗜盐菌光敏蛋白的提纯方法进行了总结和比较.细菌视紫红质(bR)的提纯主要用凝胶色谱柱的方法,而另外两种感觉视紫红质(SRⅠ和SRⅡ)要依靠高水平的蛋白质表达技术,然后再用Ni亲和层析色谱的方法提纯.由于光敏蛋白是一类具有光活性的蛋白,获得既保持其光活性又具有高纯度的蛋白质晶体是件相当困难的事.

关键词: 细菌视紫红质, 感觉视紫红质, 提纯, 光敏蛋白

Abstract: A review about purification of photosensitive protein from halophile was presented with 34 references to a survey of publications which were published on this subject.Gel chromatography was used for purifying bacteriorhodopsin basically, while Sensory rhodopsin Ⅰ and Ⅱ were purified by the Ni affinity chromatography.

Key words: bacteriorhodopsin, sensory rhodopsin, purification, photosensitive protein

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