CeO2纳米晶修饰的细菌视紫红质薄膜M态寿命的研究

doi:10.7517/j.issn.1674-0475.2002.02.126

影像科学与光化学 ›› 2002, Vol. 20 ›› Issue (2): 126-130.DOI: 10.7517/j.issn.1674-0475.2002.02.126

CeO₂纳米晶修饰的细菌视紫红质薄膜M态寿命的研究

王丽萍^1,2, 李宝芳¹, 洪广言³, 江龙¹

1. 中国科学院, 化学研究所, 分子科学中心, 北京, 100080;
2. 中国科学院, 理化技术研究所, 北京, 100101;
3. 中国科学院, 长春应用化学研究所, 稀土化学与物理开放实验室, 长春130022

收稿日期:2001-08-08 修回日期:2001-09-19 出版日期:2002-03-23 发布日期:2002-03-23
通讯作者: 王丽萍

STUDIES ON THE LIFETIME OF M-STATE IN BACTERIORHODOPSIN FILM MODIFIED WITH CeO₂ NANOCRYSTALLINE

WANG Li-ping^1,2, LI Bao-fang¹, HONG Guang-yan³, JIANG Long ¹

1. Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, P.R.China;
2. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, P.R.China;
3. Key Laboratory of Rare-earth Chemistry of Sciences, Changchun 130022, P.R.China

Received:2001-08-08 Revised:2001-09-19 Online:2002-03-23 Published:2002-03-23

摘要/Abstract

摘要： 紫膜中的蛋白质细菌视紫红质(bR)具有独特的光循环和光致变色特性 ,在分子电子学和生物电子技术领域具有广泛的潜在应用价值. 本文采用稀土纳米晶CeO₂对细菌视紫红质聚乙烯醇(bR-PVA)薄膜进行化学修饰 .研究了纳米晶对bR光循环中的重要中间态M态的寿命的影响. 发现上述稀土纳米晶可延长M态寿命, 且晶粒尺寸越小对M态寿命的影响越大. 纳米晶周围的羟基有助于阻碍席夫碱获得质子, 从而使M态寿命延长.

关键词: 细菌视紫红质, 稀土纳米晶, 化学修饰, 中间态寿命

Abstract: Bacteriorhodopsin,which consisted in the purple membrane,has unique photocycle and photochoromism properties and can be widely used in moleculer electronics and bioelectrotechnics.Bacteriorhodopsin polyvinyl alcohol (bR-PVA)films were prepared with CeO₂ nanocrystallines as chemical additives.The effect of the nanocrystallines on the lifetime of M-state,which is an important intermediate in the photocycle of bR,is investigated.It is observed that the lifetime of M-state can be prolonged by rare-earth nanocrystalline,and the lifetime of M-state increases with the decreasing size of CeO₂ nanocrystalline.Hydroxyl groups around the nanocrystallines help to prevent the Schiff base in the M-state from uptaking protons,and the decay rate is retarded and the lifetime of the M intermediate is prolonged.

Key words: bacteriorhodoposin, rare earth nanocrystalline, chemical modification, lifetime of intermediate state

中图分类号:

O644

王丽萍, 李宝芳, 洪广言, 江龙. CeO₂纳米晶修饰的细菌视紫红质薄膜M态寿命的研究[J]. 影像科学与光化学, 2002, 20(2): 126-130.

WANG Li-ping, LI Bao-fang, HONG Guang-yan, JIANG Long . STUDIES ON THE LIFETIME OF M-STATE IN BACTERIORHODOPSIN FILM MODIFIED WITH CeO₂ NANOCRYSTALLINE[J]. Imaging Science and Photochemistry, 2002, 20(2): 126-130.

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CeO₂纳米晶修饰的细菌视紫红质薄膜M态寿命的研究

STUDIES ON THE LIFETIME OF M-STATE IN BACTERIORHODOPSIN FILM MODIFIED WITH CeO₂ NANOCRYSTALLINE

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