胺基邻菲咯啉-钌(Ⅱ)反应性荧光指示剂的合成与化学固定的研究

doi:10.7517/j.issn.1674-0475.2015.06.513

影像科学与光化学 ›› 2015, Vol. 33 ›› Issue (6): 513-520.DOI: 10.7517/j.issn.1674-0475.2015.06.513

• 应用与发展 • 上一篇

胺基邻菲咯啉-钌(Ⅱ)反应性荧光指示剂的合成与化学固定的研究

胡小英, 李建雄, 刘安华

华南理工大学材料学院, 广东广州 510640

收稿日期:2015-06-16 修回日期:2015-08-17 出版日期:2015-11-16 发布日期:2015-11-16
通讯作者: 李建雄

Study on Synthesis of Amino-phenanthroline-Ru (Ⅱ) Phosphor and Its Chemical Immobilization onto PMMA

HU Xiaoying, LI Jianxiong, LIU Anhua

School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, P. R. China

Received:2015-06-16 Revised:2015-08-17 Online:2015-11-16 Published:2015-11-16

摘要/Abstract

摘要：

为减少荧光指示剂被水萃取流失,提高荧光膜的使用寿命,将5-氨基-邻菲咯啉与二(4,7-二苯基-邻菲咯啉)二氯化钌(Ⅱ)络合,合成带氨基的荧光指示剂[Ru(dpp)₂(phen-NH₂)]Cl₂,并经氨基键合到有机玻璃表面。以核磁、质谱、红外验证合成荧光指示剂的结构,研究键合条件对染色有机玻璃荧光发射的影响,监测荧光强度随水浸泡时间的变化。合成荧光指示剂的荧光发射峰值波长为570 nm,键合到有机玻璃后发射光谱红移20 nm,荧光强度受溶解氧影响,响应时间约为10 s,氧猝灭比达4。染色有机玻璃的荧光稳定性好,在50 ℃水中浸泡7个月,荧光强度下降小于1%。

关键词: 钌(Ⅱ)-荧光指示剂, 反应性荧光指示剂, 化学固定, 氧敏感荧光膜, 可靠性

Abstract:

To alleviate phosphor leaching and prolong the lifetime of fluoresce quenching sensors, a reactive phosphor [Ru(dpp)₂(phen-NH₂)]Cl₂ was synthesized through the ligation between [Ru(dpp)₂(phen-NH₂)]Cl₂ and 5-amino-phenanthroline (phen-NH₂) and immobilized to PMMA surface through covalent bonding. The structure of the synthesized phosphor and covalent bonding were verified with FT-IR, ¹HNMR, ¹³CNMR spectroscopy, UV-Vis and ES-MS. The effects of reaction conditions on the fluorescence of PMMA immobilized with phosphors were investigated and the fluorescent decay of the PMMA in 50 ℃ water was monitored. The fluorescent emission of the synthesized [Ru(dpp)₂(phen-NH₂)]Cl₂ in ethanol peaked at 570 nm and red shifted 20 nm as chemically bonded to PMMA. [Ru(dpp)₂(phen-NH₂)]Cl₂ bonded to PMMA exhibited fast response speed to dissolved oxygen and the oxygen quenching ratio I₀/I₁₀₀ was 4. After immersed in water at 50 ℃ for more than 90 days, the fluorescence intensity of PMMA bonded with [Ru(dpp)₂(phen-NH₂)]Cl₂ decreased less than 1%.

Key words: Ru(Ⅱ) phosphors, reactive phosphors, chemical bonding, oxygen sensitive fluorescent membrane, reliability

胡小英, 李建雄, 刘安华. 胺基邻菲咯啉-钌(Ⅱ)反应性荧光指示剂的合成与化学固定的研究[J]. 影像科学与光化学, 2015, 33(6): 513-520.

HU Xiaoying, LI Jianxiong, LIU Anhua. Study on Synthesis of Amino-phenanthroline-Ru (Ⅱ) Phosphor and Its Chemical Immobilization onto PMMA[J]. Imaging Science and Photochemistry, 2015, 33(6): 513-520.

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胺基邻菲咯啉-钌(Ⅱ)反应性荧光指示剂的合成与化学固定的研究

Study on Synthesis of Amino-phenanthroline-Ru (Ⅱ) Phosphor and Its Chemical Immobilization onto PMMA

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