比率型SO2荧光探针的构建及细胞成像研究

doi:10.7517/j.issn.1674-0475.2016.05.435

影像科学与光化学 ›› 2016, Vol. 34 ›› Issue (5): 435-443.DOI: 10.7517/j.issn.1674-0475.2016.05.435

比率型SO₂荧光探针的构建及细胞成像研究

熊超, 段冲, 徐俊超, 何媛媛, 张悦, 曾林涛

天津理工大学化学化工学院, 天津 300384

收稿日期:2016-07-27 修回日期:2016-08-11 出版日期:2016-09-15 发布日期:2016-09-15
通讯作者: 曾林涛
基金资助:
国家自然科学基金（21203138）和天津理工大学国家级大学生创新训练项目（201510060009）资助

Construction of a Ratiometric Fluorescent Probe for SO₂ Derivative and Its Application for Cell Imaging

XIONG Chao, DUAN Chong, XU Junchao, HE Yuanyuan, ZHANG Yue, ZENG Lintao

School of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China

Received:2016-07-27 Revised:2016-08-11 Online:2016-09-15 Published:2016-09-15

摘要/Abstract

摘要：

本文构建了一种基于苯并吲哚季铵盐结构的荧光探针用于检测SO₂衍生物。该荧光探针能够快速、灵敏、高选择性地检测HSO₃^-和SO₃^2-，并显示出颜色和荧光变化双重响应。其比率荧光强度（I₄₆₂/I₅₈₈）与HSO₃^-的浓度（0～16 μmol/L）之间具有良好的线性关系，检测限低至12 nmol/L。¹HNMR表明该探针的响应机制为1，4-亲核加成反应。激光共聚焦荧光成像结果表明，CZBI具有良好的细胞膜通透性，并且可以通过比率荧光成像实现对细胞内SO₂衍生物的监测。

关键词: 荧光探针, SO₂, 苯并吲哚, 比率, 细胞成像

Abstract:

In this study, we report a ratiometric fluorescent probe (CZBI) for sulfur dioxide (SO₂) derivatives based on the conjugate of carbazole and benzo[e]indolium, which displays colorimetric and ratiometric fluorescence dual response to HSO₃^-/SO₃^2-. The probe can quantitatively detect HSO₃^- with high specificity, fast response (within 40 s) as well as low detection limit (12 nmol/L). The fluorescence intensity ratios were linearly related to the concentrations of bisulfite ranging from 0 to 16 μmol/L. A 1, 4-nucleophilic addition reaction was proposed for the sensing mechanism of this probe, which was confirmed by ¹HNMR spectra. Fluorescence imaging of HeLa cells indicated that CZBI could be used for monitoring the intrinsically generated intracellular SO₂ derivatives in living cells by ratiometric fluorescence imaging.

Key words: fluorescent probe, SO₂, benzoindole, ratiometric, cell imaging

熊超, 段冲, 徐俊超, 何媛媛, 张悦, 曾林涛. 比率型SO₂荧光探针的构建及细胞成像研究[J]. 影像科学与光化学, 2016, 34(5): 435-443.

XIONG Chao, DUAN Chong, XU Junchao, HE Yuanyuan, ZHANG Yue, ZENG Lintao. Construction of a Ratiometric Fluorescent Probe for SO₂ Derivative and Its Application for Cell Imaging[J]. Imaging Science and Photochemistry, 2016, 34(5): 435-443.

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比率型SO₂荧光探针的构建及细胞成像研究

Construction of a Ratiometric Fluorescent Probe for SO₂ Derivative and Its Application for Cell Imaging

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