Imaging Science and Photochemistry ›› 1996, Vol. 14 ›› Issue (1): 38-43.DOI: 10.7517/j.issn.1674-0475.1996.01.38

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STUDIE ON WEAK INTERACTION BETWEEN AMP DERIVATIVE AND PYRIMIDINE BASES BY FLUORESCENCE QUENCHING──MODIFIED FORM OF STERN-VOLMER EQUATION FOR COMPETITIVE-ABSORPTION-SYSTEMS

FAN Ping1, FAN Meigong2   

  1. 1. Department OF Chemistry, Guangxi Teachers College, Nanning 530001, P. R. China;
    2. Institute of Photographic Chemistry, The Chinese Acadenyof Sciences, Beijing 100101, P. R. China
  • Received:1995-03-13 Revised:1995-10-16 Online:1996-02-20 Published:1996-02-20

Abstract: The fluorescence quenching of 8-azido-1,N6-ethene-5’-adenosine monophosphate(8-N3-E-AMP)bv thymine(T),uracil(U)and cytosine(C)at different temperature has been observed.The results indicate that the fluorescence quenching is not only due to the competitive absorption but also due to the formation of ground state complexes.The equations describing the fluorescence quenching in competitive-absorption-systems have been proposed.A modified form of Stern-Volmer equation is agreement with the results of experiments.

Key words: fluorescence quenching, competive-absorption-system, stern-volmer equation, 8-azido-1,N6-ethene-5’-AMP, pyrimdine bases