Spectroscopic Study on the Interaction of Metal Complexes of Hypocrellin A with Human Serum Albumin

doi:10.7517/j.issn.1674-0475.2010.01.1

Abstract

Abstract: Interaction of metal complexes of hypocrellin A(M-HA),such as Mg²⁺-HA, Y³⁺-HA,La³⁺-HA with human serum albumin(HSA) is studied by spectroscopic methods. The results suggest that M-HA and HSA can form 1:1 complex with moderate association constants(>2×10⁴ L/mol) at pH 7.4 and 5.0.Electron paramagnetic resonance (EPR) and fluorescence quenching experiments indicate that photoinduced electron transfer from HSA to M-HA may occur at excited state.

Key words: hypocrellin, human serum albumin, electrostatic interaction, photoinduced electron transfer, fluorescence quenching

CLC Number:

JIN Mi-mi, OU Zhi-ze, GAO Yun-yan, HAO Ping, YIN Wei-feng, LIU Li-hua. Spectroscopic Study on the Interaction of Metal Complexes of Hypocrellin A with Human Serum Albumin[J]. Imaging Science and Photochemistry, 2010, 28(1): 1-9.

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