Construction of Two-photon Excited Supramolecular Polymer Nanoparticles and Their Bioimaging Application

doi:10.7517/issn.1674-0475.190104

Abstract

Abstract: A difluoroboron β-diketonate derivative(BF₂-bisUPy)and a porphyrin derivative (Por(Pt)-bisUPy)bearing bis-2-ureido-4[1H]-pyrimidinone (bisUPy)were assembled into a supramolecular polymer by quadruple hydrogen bonding, which afforded uniformly water-dispersed supramolecular polymer nanoparticles by microemulsion method. Scanning electron microscopy shows that the obtained nanoparticles have a particle size of about 60 nm. Ultraviolet-visible absorption spectroscopy, fluorescence emission spectroscopy and lifetime decay experiments have demonstrated the occurrence of efficient energy transfer between BF₂-bisUPy and Por(Pt)-bisUPy. BF₂-bisUPy has excellent two-photon absorption performance, which can be used as an energy donor to enhance the luminescence properties of Por(Pt)-bisUPy under two-photon excitation by fluorescence resonance energy transfer(FRET). The prepared supramolecular polymer nanoparticles have strong fluorescence and phosphorescence dual-emission under two-photon excitation. The nanomaterials can enter cells and exhibit excellent biocompatibility. It also exhibits bright bioimaging with dual-emission under two-photon excitation.

Key words: supramolecular polymer, quadruple hydrogen bonding, fluorescence resonance energy transfer, two-photon, dual-emission

LIU Xiaoqin, HUANG Yuanyuan, GAO Jianfeng, CHEN Yuzhe, TONG Zhenhe(TUNG Chen-ho), WU Lizhu. Construction of Two-photon Excited Supramolecular Polymer Nanoparticles and Their Bioimaging Application[J]. Imaging Science and Photochemistry, 2019, 37(2): 136-145.

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