TDI联合血清miR-34a在CHD合并PAH患儿诊断中的应用

doi:10.7517/issn.1674-0475.220407

摘要/Abstract

摘要： 本研究探讨组织多普勒超声心动图（TDI）联合血清microRNA-34a （miR-34a）在先天性心脏病（CHD）患儿合并肺动脉高压（PAH）诊断中的价值。根据是否发生PAH将112例CHD患儿分为CHD组和CHD+PAH组，均进行TDI检查，分别测量两组患儿的TDI-Tei和TDI-Z，并通过qRT-PCR检测患儿血清miR-34a水平。结果显示，CHD组的TDI-Tei显著低于CHD+PAH组（P<0.001），CHD组的TDI-Z显著高于CHD+PAH组（P<0.001）；CHD组的血清miR-34a相对表达量显著低于CHD+PAH组（P<0.001）。相关性分析显示，TDI-Tei与TDI-Z呈负相关关系（r=－0.325，P<0.01），TDI-Tei与血清miR-34a呈正相关关系（r=0.260，P<0.01），TDI-Z与血清miR-34a呈负相关关系（r=－0.386，P<0.01）。TDI-Tei、TDI-Z和血清miR-34a联合诊断CHD合并PAH的AUC和敏感性高于单独诊断。TDI联合血清miR-34a在诊断CHD合并PAH中具有较高的价值，值得临床推广。

关键词: 先天性心脏病, 肺动脉高压, 组织多普勒超声心动图, microRNA-34a, TDI-Tei, TDI-Z

Abstract: This study explored the diagnostic value of Tissue Doppler Imaging (TDI) combined with serum microRNA-34a (miR-34a) in the diagnosis of children congenital heart disease (CHD) complicated with pulmonary arterial hypertension (PAH). According to the occurrence of PAH, all 112 CHD children were divided into CHD group and CHD+PAH group and all of them underwent TDI examination. The TDI-Tei and TDI-Z of the two groups were measured respectively, and the serum miR-34a level of the children was detected by qRT-PCR test. The results showed that the TDI-Tei of the CHD group was significantly lower than that of the CHD+PAH group(P<0.001), the TDI-Z of the CHD group was significantly higher than that of the CHD+PAH group(P<0.001), the relative expression of serum miR-34a in CHD group was significantly lower than that in CHD+PAH group(P<0.001). Correlation analysis showed that TDI-Tei was negatively correlated with TDI-Z (r=-0.325, P<0.01), TDI-Tei was positively correlated with serum miR-34a (r=0.260, P<0.01), TDI-Z was negatively correlated with serum miR-34a (r=-0.386, P<0.01). The AUC and the sensitivity of TDI-Tei, TDI-Z and serum miR-34a combined diagnosis of CHD complicated with PAH were higher than those of single diagnosis. In conclusion, TDI combined with serum miR-34a has higher value in the diagnosis of CHD complicated with PAH, and was worthy of clinical promotion.

Key words: congenital heart disease, pulmonary hypertension, tissue Doppler imaging, microRNA-34a, TDI-Tei, TDI-Z

徐姗姗, 曹仙英, 周彬. TDI联合血清miR-34a在CHD合并PAH患儿诊断中的应用[J]. 影像科学与光化学, 2022, 40(5): 1039-1044.

XU Shanshan, CAO Xianying, ZHOU Bin. Application of TDI Combined with Serum miR-34a in the Diagnosis of Children CHD Complicated with PAH[J]. IMAGING SCIENCE AND PHOTOCHEMISTRY, 2022, 40(5): 1039-1044.

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