基于CdSe/ZnS量子点构建丝裂霉素荧光检测体系的研究

doi:10.7517/j.issn.1674-0475.2016.04.346

影像科学与光化学 ›› 2016, Vol. 34 ›› Issue (4): 346-354.DOI: 10.7517/j.issn.1674-0475.2016.04.346

基于CdSe/ZnS量子点构建丝裂霉素荧光检测体系的研究

郭瑜桉, 张直峰, 申晨凡, 闫桂琴

山西师范大学生命科学学院, 山西临汾 041000

收稿日期:2016-04-18 修回日期:2016-05-20 出版日期:2016-07-15 发布日期:2016-07-15
通讯作者: 张直峰, 闫桂琴
基金资助:
山西省化学优势重点建设学科建设项目(912019)和教育部博士点联合基金项目(20111404110002)资助

A Simple Fluorescent Method for Detection of Mitomycin Based on CdSe/ZnS Quantum Dots

GUO Yu'an, ZHANG Zhifeng, SHEN Chenfan, YAN Guiqin

School of Life Science, Shanxi Normal University, Linfen 041000, Shanxi, P.R.China

Received:2016-04-18 Revised:2016-05-20 Online:2016-07-15 Published:2016-07-15

摘要/Abstract

摘要：

建立了一种基于CdSe/ZnS量子点的快速、高效的检测丝裂霉素的荧光方法。pH=7.4,反应10 min,CdSe/ZnS量子点的荧光随丝裂霉素浓度增加而猝灭,量子点的荧光强度与丝裂霉素的浓度呈良好的线性关系,适用于丝裂霉素浓度1.8~60 μmol/L,且相关系数为0.996,丝裂霉素的检测限为0.11 μmol/L,该方法相对无共存物质的干扰,并证明完全适用于丝裂霉素注射液与尿液中丝裂霉素的测定。

关键词: CdSe/ZnS量子点, 荧光, 丝裂霉素, 检测

Abstract:

In this study, we developed a simple and readily implemented fluorescent method for detection of mitomycin based on the effects of CdSe/ZnS quantum dots. At pH 7.4 and reaction time of 10 min, the fluorescence of the CdSe/ZnS quantum dots was quenched by increased concentrations of mitomycin. We used these observations to establish a linear relationship between the quenched fluorescence signal and the mitomycin concentration in the range of 1.8~60 μmol/L, with a detection limit of 0.11 μmol/L, and an correlation coefficient of 0.996. The proposed method is relatively free of interference from coexisting substances, and was shown here to be fully applicable to the detection of mitomycin in mitomycin injection and urine samples.

Key words: CdSe/ZnS quantum dots, fluorescence, mitomycin, detection

郭瑜桉, 张直峰, 申晨凡, 闫桂琴. 基于CdSe/ZnS量子点构建丝裂霉素荧光检测体系的研究[J]. 影像科学与光化学, 2016, 34(4): 346-354.

GUO Yu'an, ZHANG Zhifeng, SHEN Chenfan, YAN Guiqin. A Simple Fluorescent Method for Detection of Mitomycin Based on CdSe/ZnS Quantum Dots[J]. Imaging Science and Photochemistry, 2016, 34(4): 346-354.

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基于CdSe/ZnS量子点构建丝裂霉素荧光检测体系的研究

A Simple Fluorescent Method for Detection of Mitomycin Based on CdSe/ZnS Quantum Dots

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