Polymeric pH Sensor Film for Real-time Monitoring in Biological Culture

doi:10.7517/j.issn.1674-0475.2016.04.319

Abstract

Abstract:

Successful operation of microbioreactors is critically dependent on the monitoring and control of pH of the culture fluids. Polymeric pH fluorescent probe can be easily fabricated into films and fixed onto microbioreactors. Copolymer P(HEMA-NI) has good hydrophilicity, film-forming property and biological compatibility, along with good response and repeatability in the pH range of 6-8 in the biological fermentation tank. In order to improve the response rate of pH polymer film, the third monomer was incorporated into the polymer matrix.The results showed that the pH response rate of films decreased with the incorporation of adamantine, while with the incorporation of hydrophilic PEG into the polymer, the response rate was significantly improved. These pH sensor films showed good pH on-line monitoring performance in yeast culture.

Key words: microbioreactor, polymer, fluorescent, pH sensing, response rate

YE Wen, WANG Shuwen, PAN Mengxu, ZHAO Ping, PENG Yangfeng, ZHU Weihong. Polymeric pH Sensor Film for Real-time Monitoring in Biological Culture[J]. Imaging Science and Photochemistry, 2016, 34(4): 319-328.

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