关键词: 凝胶电泳, 构象, 照相明胶

Abstract: A careful survey and investigation of the literature references indicate that up to now SDS-PAGE method can be used to characterize the < α, α₁, α₂, β conformations γ and >γ conformations failed to be realized by this method, it is really a serious fault in full analysis of different conformations of photogelatin and in judgement of the gelatin quality based on conformation components.In general how to handle the contradiction between the crosslinkage degree (hence the pore magnitude) and the physicomechanical property of polyacrylamide gel plate is acknowledged to be the key problem in realizing the full conformation analysis.In present study a series of factors such as the relative concentration of acrylamide and the N,N-methylene-bis acrylamide and the cross-linkage gradient formation were investigated as a function of the migration distance of all conformations.An emphasis was made to observe whether the γ and > γcomponents can enter the polyacrylamide gel plate together with the other conformation components.The experimental data demonstrated that the entire conformation components ranging from < α, α₁, α₂, β, γ and > γ, without exception, can be separated and characterized with satisfactory resolution by the SDS-PAGE method improved and developed in present study.This improvement and development are of important significance for gelatin routine analysis and controlling the process parameters so as to optimize the correctly relative content of different conformations for a specialized type of gelatin products.

Key words: conformation, SDS-PAGE, photogelatm