B-藻红蛋白多层复合薄膜的静电组装及其光谱特性

doi:10.7517/j.issn.1674-0475.2009.01.57

影像科学与光化学 ›› 2009, Vol. 27 ›› Issue (1): 57-62.DOI: 10.7517/j.issn.1674-0475.2009.01.57

B-藻红蛋白多层复合薄膜的静电组装及其光谱特性

周林¹, 胡金梅^2,3, 郭祀远³

1. 广东药学院, 生命科学与生物制药学院, 广东广州510006;
2. 华南师范大学, 激光生命科学研究所, 广东广州510631;
3. 华南理工大学, 轻工与食品学院, 广东广州510640

收稿日期:2008-07-29 修回日期:2008-10-06 出版日期:2009-01-23 发布日期:2009-01-23
通讯作者: 胡金梅,E-mail:hbhjm77@163.com

Electrostatic Self-Assembly Multilayer Films of B-PE and Its Spectrum Characteristic

ZHOU Lin¹, HU Jin-mei^2,3, GUO Si-yuan³

1. College of Life Science and Biological Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, P. R. China;
2. Institute of Laser Life Science, South China Normal University, Guangzhou 510631, Guangdong, P. R. China;
3. College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, Guangdong, P. R. China

Received:2008-07-29 Revised:2008-10-06 Online:2009-01-23 Published:2009-01-23

摘要/Abstract

摘要： B-藻红蛋白是一种理想的生物光电材料.利用静电组装技术制备了聚二甲基二烯丙基氯化铵(PDDA)与B-藻红蛋白组装的多层复合薄膜.对薄膜的可见吸收光谱和荧光光谱进行了分析,结果表明:与B-藻红蛋白在溶液中的光谱特征相比,B-藻红蛋白在薄膜上的最大吸收峰由545 nm红移至553 nm,并在573 nm处产生了延迟荧光现象,且薄膜的最大吸光度和光致发射强度均与组装层数呈线性递增关系.随着薄膜组装层数的增加,倒置荧光显微镜能很好地观察到B-藻红蛋白在薄膜上出现明显的聚集体.

关键词: B-藻红蛋白, 光谱特性, 薄膜, 静电自组装

Abstract: B-Phycoerythrin was an novel bio-photoelectronic material.Multilayer films formed of PDAA and B-PE were prepared by electrostatic self-assembly method.Visible absorption spectrum and fluorescent spectrum of the multilayer films were studied.The result showed the maximum absorption peak occurred red shift from 545 nm to 553 nm,and a delayed fluorescence peak at 573 nm was observed,the maximum absorption and photoluminescence intensity increases in proportion to the assembled layers.With the increase of the self-assembly layers,Obvious aggregate of B-PE was observed on the film by Inverted Microscope Eclipse.

Key words: B-Phycoerythrin, spectrum characteristic, thin film, electrostatic self-assembly

中图分类号:

周林, 胡金梅, 郭祀远. B-藻红蛋白多层复合薄膜的静电组装及其光谱特性[J]. 影像科学与光化学, 2009, 27(1): 57-62.

ZHOU Lin, HU Jin-mei, GUO Si-yuan. Electrostatic Self-Assembly Multilayer Films of B-PE and Its Spectrum Characteristic[J]. Imaging Science and Photochemistry, 2009, 27(1): 57-62.

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B-藻红蛋白多层复合薄膜的静电组装及其光谱特性

Electrostatic Self-Assembly Multilayer Films of B-PE and Its Spectrum Characteristic

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